胃癌组织及区域淋巴结MUC1、CD44v6、nm23表达与胃癌侵袭转移及预后的关系

Expression of MUC1,CD44v6,nm23in Gastric Carcinomas and Regional Lymph Node Tissues and Their Association with Invasion,Metastasis,and Prognosis of the Tumor

背景与目的:研究证实胃癌浸润深度和淋巴结转移是多基因及其蛋白表达产物协调作用的结果,寻找与胃癌转移相关的分子生物学标志物有助于胃癌的研究。本实验旨在探讨胃癌组织及区域淋巴结中MUC1、CD44v6、nm23表达与胃癌侵袭转移及预后的关系。方法:采用SP免疫组织化学方法对110例胃癌组织及613枚区域淋巴结中的MUC1、CD44v6、nm23基因蛋白的表达进行检测。结果:①胃癌组织中的MUC1蛋白阳性表达在低分化癌组、浸润型组、T3+T4组、淋巴结转移组、Ⅲ～Ⅳ期组、生存期<5年组分别为84.6%、88.1%、87.3%、91.7%、94.4%、95.5%,CD44v6分别为79.5%、74.6%、79.4%、81.7%、87.0%、87.9%,nm23分别为38.5%、32.2%、30.2%、25.0%、25.9%、18.2%。MUC1和CD44v6表达低分化癌组显著高于高、中分化癌组(78.9%vs57.7%),浸润型组高于局限型组(72.6%vs54.9%),T3+T4组高于T2组(72.3%vs46.8%),淋巴结转移组高于无淋巴结转移组(68.0%vs46.0%),TNMⅢ～Ⅳ期组高于Ⅰ～Ⅱ期组(67.9%vs44.6%),生存期<5年组高于≥5年组(59.1%vs31.8%),各组间差异均具有显著性(P<0.01或P<0.05)。而nm23除分化程度、Borrmann分型不同的两组之间差异无显著性外,T3+T4组低于T2组(51.1%),有淋巴结转移组低于无淋巴结转移组(56.0%)。

BACKGROUND &OBJECTIVE:The invasion depth and lymph node metastasis result from the poly g enes and their protein expression i n g astric carcinoma.The key of the basic and cl inical research of the g astric carci noma is to find out the related molecular bio log y marker.This study was desig ned to investig ate the relationship betwe en the expression of MUC1,CD44v6,nm23in g astric carcinomas and reg ional lym ph node tissues and invasion,metast asis ,and prog nosis of the tumor.METHODS :The expression of MUC1,CD44v6,and nm23in 110advanced g astric carc inomas and 613reg ional lymph node specimens were examined by immunohi stochemical technique.The results was judg ed by the criterion of the positive or the neg ative expression.RESULTS :(1)The expression of MUC1(84.6%,88.1%,87.3%,91.7%,94.4%,a nd95.5%,respectively)and CD44v6(79.5%,74.6%,79.4%,81.7%,87.0%,and 87.9%,respectively)in g astric cancinoma tissues in the g roups of low differentiated adenocarcinoma ,infiltrated type,T 3 +T4,lymph node metastasis ,stag e Ⅲ-Ⅳ,and survival <5years were sig nific antly hig her than those in the g roups of hig h-midd le differentiated adenocarcinoma,limited type,T1+T2,no lymph node metastasi s ,stag e Ⅰ-Ⅱ,and survival ≥5years(P<0.01or P<0.05);while the expression of nm23was contrary(P<0.01or P<0.05)except for the g roups of differentia tion and Borrmann type.(2)The expression of MUC1and CD44v6in the l ymph node metastasis g roup of the g astric carcinoma (94.7%and 89.4%,respectively)were remarkably hig her than those in the no lymph node metast asis g roup(19.6%and 19.6%,respectively)(P<0.01);while the expression of nm23was con trary(16.8%versus 74.9%,P<0.01).(3)The 5-year survival rates of the patients in MUC1and CD44v6positive expression g roup(13.0%and 15.4%,respectively)in the reg ional lymph nodes of g asric carci noma were sig nificantly lower than t hose in the neg ative expression g roup(100%and 62.1%)(P<0.01);while the5-year survival rate of the patients in nm23positive expression g roup wa s contrary(70.0%versus 4.0%,P<0.01).CONCLUSION:The hig h expression of MUC1,CD44v6and low expression of nm23were related to the invasion,metastasis ,and prog nosis of the g as tric carcinoma.The detection of MUC1,CD44v6,and nm23can be useful for the diag nosis and treatment of advanced g astric carcinoma.