叶底珠抑菌活性内生真菌的筛选及鉴定

Isolation and Identification of Endophytic Fungi with Antimicrobial Activity from Securinega Suffruticosa

内生真菌存在于植物中,代谢产物十分丰富,具有多种生物活性,是新化合物、新药源的潜在资源。叶底珠通常根部入药,用于治疗风湿腰痛、四肢麻木和小儿疳积等疾病,对神经衰弱、急性肝损伤等也有一定的疗效。本文主要从叶底珠中分离筛选具有抑菌活性的内生真菌,并对其进行鉴定。采用平板培养法从叶底珠中分离内生真菌,通过滤纸片扩散法考察这些内生真菌的抑菌活性。对具有良好抑菌效果的内生真菌进行培养观察和ITS序列测定,鉴定其归属。从健康植株中分离到41株内生真菌,其中2个菌株的提取液对白色念珠菌的抑制效果最好,并发现其对另外7种病原菌具有不同程度的抑制作用。通过鉴定,确定菌株G11为球毛壳菌(Chaetomium globosum),菌株D11为枝细枝孢菌(Cladosporium ramotenellum)。从叶底珠中分离到2株具有较好抑菌作用的内生真菌,这些具有抑菌作用的内生真菌可用于生物制药和农业产业,值得进一步研究。

Endophytic fungi with antibacterial activity were isolated,screened from Securinega suffruticosa,and identified.These fungi were isolated from S.suffruticosa by plate culture method,and their antibacterial activity was examined by filter paper diffusion.The endophytic fungi with good antimicrobial activity were sequenced by ITS,and their corresponding genera were identified.A total of 41 endophytic fungi were isolated from healthy plants.Among these isolates,the extracts of 2 strains had the best inhibition effect on Candida albicans,and they had different inhibitory effects against the other seven pathogens.Strain G11 was identified as Chaetomium globosum,whereas strain D11 was identified as Cladosporium ramotenellum.Two endophytic fungi with good antimicrobial activity were isolated and screened from S.suffruticosa.These endophytic fungi can be used in biopharmaceutical and agricultural industries and merit further study.