姜黄素对TPA诱导的银屑病小鼠模型血清ASO和IL-8水平的影响研究

Impact study of curcumin on ASO and IL-8 level changes in serum of psoriatic mice model induced by TPA

目的探究姜黄素对佛波酯(phorbol ester,TPA)诱导的银屑病小鼠模型血清抗链球菌溶血素"O"(antistreptolysin"O",ASO)和白细胞介素-8(Interleukin-8,IL-8)水平的影响。方法选取132只SCID小鼠,将其分为3组。正常组43只,模型组46只,干预组43只。采用佛波酯诱导,将模型组及干预组小鼠建立银屑病模型。3组小鼠在相同环境下饲养,予干预组小鼠姜黄素,0.02g/100 g溶于3 mL双蒸水中灌胃,每天1次,2周为1个疗程。治疗结束后,对3组小鼠进行血清抗链球菌溶血素"O"(ASO)和白细胞介素-8(IL-8)水平进行比较。结果治疗前,与正常组小鼠血清抗链球菌溶血素"O"阳性率比较,干预组及模型组显著升高,差异有统计学意义(P<0.05);治疗后,干预组显著低于模型组,差异有统计学意义(P<0.05);治疗前,与正常组IL-8比较,干预组及模型组显著升高,差异有统计学意义(P<0.05);治疗后,干预组小鼠白细胞介素-8显著低于模型组,差异有统计学意义(P<0.05)。结论姜黄素可有效降低银屑病小鼠模型血清抗链球菌溶血素"O"(ASO)和白细胞介素-8(IL-8)水平,改善银屑病小鼠的临床不良症状,是治疗银屑病的有效药物。

Objective To explore the impact of curcumin on antistreptolysin“O”(ASO)and interleukin 8 (IL-8)level changes in serum of psoriatic mice model induced by phorbol ester(TPA).Methods 132 SCID mice were selected and divided into three groups.43 mice in normal group, 46 mice in model group,and 43 mice in intervention group.The mice of model group and intervention group were set up psoriasis model induced by TPA. Mice of the three groups were bred in the same environment,curcumin was injected in the intervention group mice,0.02 g/100 g,once a day,two weeks for a period of treatment.ASO and IL-8 level were compared amorg the three groups of mice after treatment.Results Before the treatment,compared with the normal group,positive rate of ASO in model group and intervention group increased significantly,and the difference was statistically significant (P<0.05 );after treatment,the level of intervention group was significantly lower than model group,and the difference was statistically significant (P<0.05 );before the treatment,compared with the normal group,IL-8 level in intervention group and model group increased significantly,and the difference was statistically significant (P<0.05 );after treatment,level of the intervention group was significantly lower than model group,and the difference was statistically significant (P<0.05).Conclusion Curcumin could effectively reduce ASO and IL-8 level in serum of psoriatic mice model, improve the clinical symptoms of psoriasis mice,it is an effective drug for treatment of psoriasis.