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Selective Anti-Hepatoma Treated with Titanium Oxide Nanoparticles in vitro 被引量:5

Selective Anti-Hepatoma Treated with Titanium Oxide Nanoparticles in vitro
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摘要 Effects of titanium oxide (TiO 2) nanoparticles on Bel-7402 human hepatoma cells and L-02 human hepatocytes at different times were observed.Using cell culture,cell growth curves of Bel-7402 cells and L-02 cells treated with TiO 2 nanoparticles were examined by MTT assay,and the cellular ultrastructure was observed by an analytical transmission electron microscope (ATEM).It is found that OD value of Bel-7402 cell treated with TiO 2 nanoparticles for 48-144h is obviously lower than that of control group (p<0.01).However the growth curve of L-02 cells is almost not affected by TiO 2 nanoparticles.ATEM and energy dispersive X ray (EDX) analyses show that there are obvious vacuoles increased heterolysosome,and particles with high electron density which are confirmed to be TiO 2 nanoparticles in Bel-7402 cytoplasm.More interestingly,it is alse found that TiO 2 nanoparticle obviously inhibits the proliferation of hepatoma cells by altering lysosome activity and destroying cytoplasm structure.The inhibition on proliferation of hepatocytes by TiO 2 nanoparticles is much slighter.The results demonstrate that TiO 2 nanoparticle has different killing effects on cancer cell and normal cell. Effects of titanium oxide (TiO 2) nanoparticles on Bel-7402 human hepatoma cells and L-02 human hepatocytes at different times were observed.Using cell culture,cell growth curves of Bel-7402 cells and L-02 cells treated with TiO 2 nanoparticles were examined by MTT assay,and the cellular ultrastructure was observed by an analytical transmission electron microscope (ATEM).It is found that OD value of Bel-7402 cell treated with TiO 2 nanoparticles for 48-144h is obviously lower than that of control group (p<0.01).However the growth curve of L-02 cells is almost not affected by TiO 2 nanoparticles.ATEM and energy dispersive X ray (EDX) analyses show that there are obvious vacuoles increased heterolysosome,and particles with high electron density which are confirmed to be TiO 2 nanoparticles in Bel-7402 cytoplasm.More interestingly,it is alse found that TiO 2 nanoparticle obviously inhibits the proliferation of hepatoma cells by altering lysosome activity and destroying cytoplasm structure.The inhibition on proliferation of hepatocytes by TiO 2 nanoparticles is much slighter.The results demonstrate that TiO 2 nanoparticle has different killing effects on cancer cell and normal cell.
作者 曹献英
出处 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2003年第1期52-54,共3页 武汉理工大学学报(材料科学英文版)
基金 FundedbytheNationalNaturalScienceFoundationofChina (No :39770 2 2 5 )
关键词 titanium oxide NANOPARTICLE hepatoma cells titanium oxide nanoparticle hepatoma cells
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