Cr^(3+),Cr^(6+)及其复合污染对狐尾藻的毒害作用

Toxic effects of Cr^(3+), Cr^(6+) and their combined pollution on Myriophyllum verticillatum

采用急性毒害水培法,研究比较了不同浓度的Cr^(6+),Cr^(3+)单一及复合处理对狐尾藻不定根和叶片生理生态的影响。实验结果表明:1)当浓度≤1mg·L^(-1)时,Cr^(3+)对不定根的生长表现出促进作用,数量增加,长度增长。而Cr^(6+)及复合处理系列对不定根却表现出强烈的抑制作用,在高浓度时甚至使其生长停止;2)叶绿素含量在1mg·L^(-1)Cr^(3+)培养液中达到最大值,随Cr^(6+)及复合处理浓度的增大而呈连续下降趋势;3)可溶性蛋白含量在三种处理方式中都表现出抛物线形式,复合处理组在1mg·L^(-1)时使蛋白含量出现高峰;4)细胞内超氧阴离子产生速率,膜脂过氧化水平与污染浓度和时间呈显著正相关;5)随着处理浓度的增加,SOD,OD,CAT活性先升后降,但其高峰分别出现在处理浓度为1mg·L^(-1),10mg·L^(-1),5mg·L^(-1)时;6)研究证实,Cr^(6+),Cr^(3+)复合处理对狐尾藻的毒害作用较单一Cr^(6+),Cr^(3+)显著,二者表现出协同作用,而其中Cr^(6+)毒性大于Cr^(3+)。

The paper briefly dealt with the toxic effects of Cr^(6+), Cr^(3+) and their combined pollution on the physiology of roots and leaves of high aquatic plant—Myriopllum verticillatum. The results show that Cr^(3+) could stimulate the growth of root when the treated concentration below 1 mg·L^(-1), while Cr^(6+) and the combination treatment show significant inhibition to the growth of root. The highest concentration of chlorophyll gained when treated with Cr^(3+) at concentration of 1 mg·L^(-1). The concentration of chlorophyll decreased as the increase of Cr^(6+) concentration. The concentration of soluble protein increased at first and decreased finally as the promotion of concentration of Cr^(6+), Cr^(3+) and their combined treatment. The same effects also took place on the activities of SOD, POD and CAT. The peak of concentration of protein occurred at the combination treatment of 1 mg·L^(-1). The results suggest Cr^(6+) and Cr^(3+) have corroborative pollution effect to the M. verticillatum, and Cr^(6+) is more poisonous than the Cr^(3+).