经皮附睾、睾丸穿刺取精卵浆内显微注射治疗阻塞性无精子症

Percutaneous epididymal and/or testicular sperm aspiration and intracytoplasmic sperm injection in the treatment of obstructive azoospermia

目的 :应用经皮附睾、睾丸穿刺取精术 (PETSA) ,结合单精子卵浆内显微注射 (ICSI)技术治疗阻塞性无精子症。方法 :对 8对不孕症夫妇 ,男性采用经皮附睾、睾丸穿刺取精术 ;女方采用长方案和短方案超排卵 ,对分裂中期 (MⅡ )的卵母细胞行ICSI。结果 :9个周期中 ,获MⅡ 4 6个 ,均行ICSI,其中正常受精 33个 ,形成胚胎 2 2个 ,3例临床妊娠。结论

Objective:To treat obstructive azoospermia by percutaneous epididymal,testicular sperm aspiration(PETSA) and intracytoplasmic sperm injection(ICSI).Methods:Eight couples were performed PETSA and ICSI.The male were treated by PETSA to obtain normal spermatozoon.Ovarian stimulation was achieved by the long or short protocol.All metaphas Ⅱ (MⅡ) oocytes were selected for ICSI.Results:Forty six MⅡ oocytes were injected in 9 cycles;33 fertilized;and 22 embryos were implanted.Three couples achieved clinical pregnancy.Conclusions:PETSA and ICSI are effective approaches in treatment of obstructive azoospermia.