摘要
目的 研究IL 1的Ⅰ型受体 (IL 1RⅠ )在大鼠颈动脉体内的表达和亚细胞定位。 方法 灌流固定的正常大鼠颈动脉体组织 ,用冰冻置换法包埋 ,超薄切片 ,胶体金免疫组织化学染色 ,电镜下观察。 结果 IL 1RⅠ样免疫反应阳性产物主要存在于主细胞 ,胶体金颗粒分布在胞膜、胞浆和胞浆中的细胞器及细胞核。支持细胞和毛细血管内皮细胞的胞浆中也可见少量免疫胶体金颗粒。 结论 IL 1RⅠ在大鼠颈动脉体特别是主细胞中有较强表达 ,此结果为进一步研究颈动脉体作为细胞因子化学感受器的可能性提供形态学基础。
Objective To delineate the cellular and subcellular localization of IL-1 receptor type Ⅰ(IL-1RⅠ) in the carotid body of the rat. Methods Carotid body tissues,from the normal SD rat fixed by perfusion,were embedded with freeze substitution technique.The ultra-thin sections were cut,immunohistochemically stained for IL-1R with immuno-colloid gold method,and observed under electron microscope. Results IL-1RⅠ mainly expressed in the principal cells(also known as type Ⅰ cells or glomus cells).Immunogold granules were seen to be distributed in the cellular membrane and cytoplasm including some organelles within it,as well as in the nuclei.In addition,a lower density of gold particles was found in the cytoplasm of sustentacular cells (type Ⅱ cells) and endothelial cells of the capillaries as well.Conclusion IL-1RⅠ is strongly expressed in the carotid body,especially in principal cells.The present result offers a morphological basis for further study on the role of the carotid body being as a cytokine chemoreceptor.
出处
《解剖学报》
CAS
CSCD
北大核心
2004年第1期9-12,共4页
Acta Anatomica Sinica
基金
国家自然科学基金资助课题 ( 3 0 2 70 44 1
3 983 0 13 0 )
