PureSperm离心法在宫腔内人工授精周期中优选精子的应用

Separation of High-quality Sperm by PureSperm Centrifugation Applied to Intrauterine Insemination Cycles

目的 :探讨PureSperm离心法在宫腔内人工授精 (IUI)周期中优选精子的效果。 方法 :采用PureSperm单、双梯度离心法及Percoll双梯度离心法分别处理精液 ,比较 3种方法的处理效果 ,并将处理后的精子悬液施行夫精IUI。 结果 :单梯度PureSperm较双梯度的PureSperm及Percoll洗涤后精子密度显著增加 (P <0 .0 1) ,双梯度PureS perm及Percoll离心法两者差异无显著性 (P >0 .0 5 ) ,3种方法在洗涤后精子活率、畸形率及妊娠率比较差异均无显著性 (P >0 .0 5 )。洗涤后正常形态的精子数量明显增加 ,白细胞、上皮细胞及细胞碎片等非精子成分明显减少。 结论 :PureSperm离心法是一种高效、简便、安全的IUI精液处理方法。

Objective: To separate high-quality sperm by PureSperm centrifugation applied to intrauterine insemination (IUI) cycles.Methods: We compared the separate results after washing the semen with one-layer and two-layer PureSperm gradient centrifugation methods and two-layer Percoll gradient centrifugation method, and used the recovered high-quality sperm for IUI. Results: The density of the sperm washed with one-layer PureSperm centrifugation method was significantly higher than that washed with two-layer Percoll and two-layer PureSperm centrifugation methods(P< 0.01), but there were no differences in all the results between the use of two-layer Percoll and two-layer PureSperm(P> 0.05). No significant differences in the motility, teratozoospermia and IUI results were found when the three methods were used for sperm preparation(P> 0.05). The percentage of morphologically normal sperm was markedly increased, and the non-sperm components such as leucocytes, epithelial cells and cellular fragments were significantly reduced after washed by the two methods. Conclusion: PureSperm centrifugation is a safe, efficient and easy method for separating high-quality sperm on intrauterine insermination cycles.