TK基因突变试验——琼脂平皿法与微孔平板法的比较

Thymidine kinase gene mutation assay- comparison of microwell method and soft agar method

目的 TK(thymidinekinase)基因突变试验可采用琼脂平皿法或微孔平板法 ,本研究旨在比较两种方法的优劣 ,为选择一种适于在国内推广的方法提供依据。方法 同时使用琼脂平皿法和微孔平板法对四种化学物 (MMS、EMS、MMC、KCl)的致突变性进行检测与评价。结果 两种方法检出三种诱变剂的结果基本一致 ,但对KCl而言 ,琼脂平皿法检测结果为阴性 ,而微孔平板法检测结果为阳性。结论 就目前实验结果来看 ,宜推广琼脂平皿法在国内的应用。最终结论尚需增加更多受实物后才能确定。

Objective TK gene mutation assay was developed for a long time. Two methods are used in TK locus mutation assay, i. e. microwell method and soft agar method. Two methods were compared in this study so that to find out the better one to be used in China. Methods in this study, two methods (microwell method and soft agar method) were used for detecting and evaluating the mutagenesis of three positive mutagens, i.e. MMS, EMS, and MMC, and a non-mutagen KCl. Results Two methods was similar in results for three mutagens. For KCl, however, a negative result was showed in soft agar method while a positive result was showed in microwell method.Conclusion according to the present study, it is recommended that the soft agar method should be popularized in China.