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Isolation and Characterization of Novel Microsatellite Markers in Walnut (<i>Juglans regia</i>L.)

Isolation and Characterization of Novel Microsatellite Markers in Walnut (<i>Juglans regia</i>L.)
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摘要 Thirteen microsatellite markers were isolated and characterized from Juglans regia L. Successful polymorphism of the 13 primer pairs was observed in various genotypes of J. regia. The number of polymorphic alleles ranged from 2 to 4 (with an average of 4.35). The polymorphic information content values ranged from 0.47 to 0.88 (with an average of 0.69). TC/AG and GAA/CTT class of repeats were the most abundant di-nucleotide and tri-nucleotide repeats, respectively. Thirteen microsatellite markers were isolated and characterized from Juglans regia L. Successful polymorphism of the 13 primer pairs was observed in various genotypes of J. regia. The number of polymorphic alleles ranged from 2 to 4 (with an average of 4.35). The polymorphic information content values ranged from 0.47 to 0.88 (with an average of 0.69). TC/AG and GAA/CTT class of repeats were the most abundant di-nucleotide and tri-nucleotide repeats, respectively.
出处 《American Journal of Plant Sciences》 2014年第3期409-415,共7页 美国植物学期刊(英文)
关键词 Isolation MICROSATELLITE JUGLANS regia L. Polymorphism SSR Markers Isolation Microsatellite Juglans regia L. Polymorphism SSR Markers
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