摘要
The aim of this study was to evaluate the metabolic adaptations due to the inclusion of pasture in the diet of dairy cows fed on a total mixed ration (TMR) ad libitum during early lactation. Multiparous cows (n=18) were used in a randomized complete block design and were randomly assigned according to parity, BW and BCS to one of two feeding strategies from calving to 60 DIM: 1) cows fed TMR ad libitum (without access to pasture;100% TMR) and 2) cows fed on a mixed system with pasture grazing (6 h of access to paddock in one grazing session, 8:00 to 14:00 h) and supplemented with 50% of ad libitum TMR (Pasture Group, PG). At 61DIM, TMR fed cows were assigned without an adjustment period to a similar feeding and management routine than PG group (Post-TMR), while PG cows remained in their original routine throughout the experiment. Thus, at 61DIM and thereafter, both, PG and Post-TMR cows grazed a second-year pasture and were supplemented with 50% TMR (DM basis). Milk production was determined daily until 80 DIM, and cow BCS and BW were registered and blood samples and liver biopsies were obtained one week before and one week after dietary change (-1 to +1 wk;+55 and +69 DIM). Milk yield, BW and BCS did not differ between treatments but decreased or tended to decreased from -1 to +1 wk only in Post-TMR cows. Serum IGF-1 tended to increase in Post-TMR cows. Hepatic expression of IGFBP5 and IGFBP6 mRNA, were greater while IGF1 and IGFBP3 mRNA tended to be greater for Post-TMR than PG cows. Hepatic expression of IGF1, IGFBP5 and IGFBP6 mRNA increased from -1 to +1 wk only in Post-TMR cows. Expression of ACADVL and PDH1A mRNA had a 2-fold increase in both groups from wks -1 to +1. The results confirm that changes in feeding strategy without an adaptation period modified animal metabolism. The inclusion of grazing to cows that were fed TMR during early lactation, increased IGF-1 concentrations and modified hepatic expression of genes related with IGF system and fatty acid metabolism indicating redistribution of nutrients and energy towards maintenance requirements (increased due to walking and grazing activity) in detriment of milk production.
The aim of this study was to evaluate the metabolic adaptations due to the inclusion of pasture in the diet of dairy cows fed on a total mixed ration (TMR) ad libitum during early lactation. Multiparous cows (n=18) were used in a randomized complete block design and were randomly assigned according to parity, BW and BCS to one of two feeding strategies from calving to 60 DIM: 1) cows fed TMR ad libitum (without access to pasture;100% TMR) and 2) cows fed on a mixed system with pasture grazing (6 h of access to paddock in one grazing session, 8:00 to 14:00 h) and supplemented with 50% of ad libitum TMR (Pasture Group, PG). At 61DIM, TMR fed cows were assigned without an adjustment period to a similar feeding and management routine than PG group (Post-TMR), while PG cows remained in their original routine throughout the experiment. Thus, at 61DIM and thereafter, both, PG and Post-TMR cows grazed a second-year pasture and were supplemented with 50% TMR (DM basis). Milk production was determined daily until 80 DIM, and cow BCS and BW were registered and blood samples and liver biopsies were obtained one week before and one week after dietary change (-1 to +1 wk;+55 and +69 DIM). Milk yield, BW and BCS did not differ between treatments but decreased or tended to decreased from -1 to +1 wk only in Post-TMR cows. Serum IGF-1 tended to increase in Post-TMR cows. Hepatic expression of IGFBP5 and IGFBP6 mRNA, were greater while IGF1 and IGFBP3 mRNA tended to be greater for Post-TMR than PG cows. Hepatic expression of IGF1, IGFBP5 and IGFBP6 mRNA increased from -1 to +1 wk only in Post-TMR cows. Expression of ACADVL and PDH1A mRNA had a 2-fold increase in both groups from wks -1 to +1. The results confirm that changes in feeding strategy without an adaptation period modified animal metabolism. The inclusion of grazing to cows that were fed TMR during early lactation, increased IGF-1 concentrations and modified hepatic expression of genes related with IGF system and fatty acid metabolism indicating redistribution of nutrients and energy towards maintenance requirements (increased due to walking and grazing activity) in detriment of milk production.
