卵巢组织体外培养及冻融技术的研究进展

Research progress of in vitro culture and freeze-thaw technology for ovarian tissue

癌症患者治疗前行卵巢组织冷冻保存、待其病情稳定后,重新进行卵巢组织移植,可使其保持女性的正常生殖内分泌功能,并能够进行正常生育。目前常用的冷冻方案包括慢速程序化冷冻和玻璃化冷冻,其中慢速程序化冷冻是卵巢组织冷冻的标准方法,但近年来玻璃化冷冻由于其方便、高效,越来越受到关注。卵巢组织冷冻保存作为癌症患者保留生育能力的一种选择具有广泛的应用前景。虽然卵巢组织移植已用于临床,并有成功分娩的报道,但卵巢组织体外培养技术尚处于研究阶段,仍不够成熟。本文旨在对卵巢组织体外培养及冻融技术的方法改进以及现阶段的研究情况作一综述。

Cryopreserving ovarian tissue before the cancer treatment and ovarian tissue retransplantation can maintain the normal reproductive endocrine function and fertility. At present, frequently used freezing programs include slow programmed freezing and vitrification freezing. Slow programmed freezing is the standard protocol, while vitrification has attracted more and more attention due to its convenience and efficiency in recent years. Ovarian tissue transplantation has been used clinically, and there are reports of successful delivery. Cryopreservation of ovarian tissue has a broad application prospect in clinic as a choice of fertility preservation for cancer patients. But the in vitro culture technology of ovarian tissue is immature, and it is still in the research stage. This article aims to review the improvement of the methods of in vitro culture for ovarian tissue and the freeze-thaw technology, as well as the current research.