3D生物打印在肝细胞癌治疗中的研究进展

Advancement in the research of 3D bioprinting in the treatment of liver cancer

肝细胞癌(hepatocellular carcinoma,HCC)是一种高度致命性恶性肿瘤,具有极高的发病率和死亡率。研究HCC细胞的生物学特性是寻找HCC治疗新方法的重要环节。传统的细胞培养技术无法提供有关HCC细胞在三维(three dimension,3D)空间内生长和扩散的信息,而生物3D打印技术提供了一种研究HCC细胞功能特性的新方法。随着3D细胞培养技术的发展,越来越多的研究集中在探索细胞在三维环境中的功能和行为方面,尤其是对肝脏肿瘤这类富含细胞组织和血管的复杂组织模型。本文通过综述3D生物打印技术在HCC和其他各种人类癌细胞系中的研究成果,讨论了需要在HCC及其他肿瘤细胞的3D生物打印环境中研究的细胞功能,以期为研究人员提供信息,帮助其更准确地复制体内肿瘤细胞的生长环境,扩大应用场景,并进一步探索恶性肿瘤的治疗方法。此外,3D生物打印技术被认为是一种有前途的药物开发工具,能为抗肿瘤药物的药理学研究提供更具临床意义的模型,从而更好地揭示肿瘤对药物的敏感度和耐药性,减少动物实验的需要,为实现精准医疗提供更多可能。

Hepatocellular carcinoma(HCC)is a highly lethal cancer with significant incidence and mortality rates.The study of the biological features of liver cancer cells is critical for the development of novel treatment strategies for HCC.While traditional cell culture techniques fail to provide information on the growth and diffusion of cells in a threedim-ensional space(3D),3D bioprinting technology provides a new method to study the functional characteristics of HCC cells.With the development of 3D cell culture technology,researches are largely focused on exploring the function and behavior of cells in a three-dimensional environment,particularly in complex tumor models like liver tumors that comprise intricate cellular tissues and blood vessels.This article discussed about the cellular functions that need to be studied in the 3D bioprinting environment of HCC and other tumor cells by reviewing the research progress of 3D bioprinting in HCC and various other cancer cell lines,aiming to help researchers replicate the in vivo growth environment of tumor cells more accurately,expand application scenarios,and further explore cancer treatment methods.In addition,3D bioprinting technology is considered a promising tool for drug development,providing a more clinically relevant model for the pharmacological study of anti-cancer drugs,better revealing the drug sensitivity and resistance of tumors,reducing the need for animal experiments,and providing more possibilities for precision medicine.