应用DBA标记研究胃粘膜肠上皮化生与胃癌的关系

STUDY ON RELATIONSHIP BETWEEN GASTRIC CARCINOMA AND INTESTINAL METAPLASIA USING DBA LEBELLING

许多学者认为胃粘膜肠上皮化生(肠化)与胃癌的发生有密切关系,但少数认为肠化是常见病变,其发生率和病变的广度均随年龄增长而增加与胃癌发生无明显关系。晚近,免疫病理学的发展促进了这方面的研究,板桥正幸等应用癌胚抗原及胃肠癌抗原的单克隆抗体标记早期胃癌组织,发现胃癌与不完全型肠化关系密切。本文应用ABC(Avidin—Biotin-Peroxidase Complex)酶标技术和双重染色方法,观察胃粘膜肠化之杯状细胞中双花扁豆凝集素(DolichosBiflorus Agglutinin简称DBA)受体的分布,以探索胃癌和肠化的关系。

Distribution of Dolichos Biflorus Agglutinin(DBA)receptor was studied in 188 specimensof gastrointestinal mueosa using Avidin-Biotin-Peroxidase Complex(ABC)enzyme-labelledtechnic.Among which 83 sections were stained with HID,and 25 were double Stained withDBA and HID.The positive rate of DBA labelling for duodenal and colonic mueosa were15.00%and 90.91%respectively.The positive rate of DBA labelling was 26.74%forgastritis with intestinal metaplasia(IM)and 73.58%for IM surrounding carcinoma.Comparing DBA labelling with HID staining,we found that some goblet cells were bothpositive to DBA and HID,while others were only positive to DBA or HID.The markeddifference of DBA distribution in goblet cells of IM between earcinoma and gastritissuggests that colonic type IM may be related to the gastric carcinoma.Furthermore,it ispresumable that the goblet cells carrying DBA receptor might be the precancerous lesion.The examination of DBA distribution in gastroscopic biopsy specimens is considered to behelpful for following up patients suffered from IM.