罗丹明6GDN——氩离子激光对培养胃癌细胞(SGC-7901)的光辐射效应

PHOTOSENSITIZATION OF RHODAMINE 6GDN——Ar+ LASER ON HUMAN STOMACH CARCINOMA CELLS (SGC-7901) IN CULTURE

光辐射治疗(Photoradiation therapy,PRT)是利用某些有光敏特性的化合物结合光照进行恶性肿瘤治疗的一种实验性方法。Schwartz首先以血红蛋白制备了非金属卟啉衍生物(HpD);Lipson等证明此化合物能集中在肿瘤组织中,于1975年进入临床试用。

Rhodamine 6GDN,a fluorescent dye,was found to inhibit cultured human gastriccarcinoma cell(SGC-7901)growth in vitro by 50%at 6.5ng/ml within 24hr of exposure.The use of 1μg/ml Rhodamine 6GDN,a non-growth inhibitory concentration,incombination with lower power Argon laser radiation produced a remarkable cytotoxicityon SGO-7901 cel1s.After 1-4 hr of radiation,the mitotic cells were decreased significantlyand the morphologic changes such as disarrangement and aberrant mitosis of cells,pyknosis,coagulation and fragmentation of the nuclei were observed.Twenty-four hourslater,98%of cells treated with Rhodamine 6GDN-Arlaser were killcd.A study of~8H-TdR incorporation into DNA of cells showed that the%inhibition of the precursorincorporation was 66%when the cells were pretreated for 24 hr with Rhodamine 6GDN andthen radiated with Arlaser,whereas treatment with Rhodamine 6GDN alone decreasedthe 8-TdR incorporation by less than 40%.Using colony formation method,the viabilityof Rhodamine 6GDN on the normal hamster lung cells(V79)and malignant cells SGO-7901 was measured.The results showed that the compound possessed a selective cytotoxiceffect on malignant cells.