酶催化合成大豆甾醇油酸酯的工艺研究

Enzymatic synthesis of soybean sterol oleate

以大豆甾醇和油酸为原料,在酶的催化下合成大豆甾醇油酸酯,采用高效液相色谱对产物进行定性定量分析,通过单因素实验考察催化剂脂肪酶的种类和用量、醇酸摩尔比、反应温度和反应时间等对大豆甾醇油酸酯产率的影响,并通过正交实验优化大豆甾醇油酸酯的合成工艺条件。采用红外光谱对产物进行了表征。结果表明:大豆甾醇油酸酯的最佳合成工艺条件为催化剂N435脂肪酶用量6%(以大豆甾醇和油酸的总质量计)、醇酸摩尔比1∶1、反应温度50℃、反应时间30 h、异辛烷用量10 mL(大豆甾醇为1 mmol时),在最佳条件下大豆甾醇油酸酯产率为86.51%;红外表征说明合成的产物为大豆甾醇油酸酯。

Soybean sterol oleate was synthesized from soybean sterol and oleic acid catalyzed by enzyme.The product was qualitatively and quantitatively analyzed by HPLC chromatography.The effects of the type and amount of lipase,the molar ratio of soybean sterol to oleic acid,reaction temperature and reaction time on the yield of soybean sterol oleate were investigated by single factor experiment.The optimal synthesis process of soybean sterol oleate was obtained by orthogonal experiment.The product was characterizated by infrare spectrum.The optimal synthesis conditions of soybean sterol oleate were obtained as follows:with N435 lipase catalyst,molar ratio of soybean sterol to oleic acid 1∶1,catalyst dosage 6%(based on total mass of soybean sterol and oleic acid),reaction temperature 50℃,reaction time 30 h,isooctane dosage 10 mL(1 mmol of soybean sterol).Under these conditions,the yield of soybean sterol oleate was up to 86.51%.The infrared characterization results showed that the synthesized product was soybean sterol oleate.