摘要
OBJECTIVE:To identify,rapidly and accurately,the chemical composition of the traditional Uighur formulation Baixuan Xiatare(BXXTR-FU).METHODS:We investigated if application of three-stage infrared(IR)spectroscopy enabled identification of the main chemical constituents(and their origins)in BXXTR-FU.RESULTS:The characteristic peaks of herbal material(s)and BXXTR-FU were assigned.In Fourier transform-IR(FT-IR)spectroscopy of BXXTR-FU,peaks at1616 and 1605 cm-1 of BXXTR-FU were considered to denote anthraquinones and their derivatives;1066 cm-1 was regarded as the characteristic absorption peak of resin glycosides.In second-derivative IR(SD-IR)spectroscopy,the main carbonyl types of BXXTR-FU in the range 1743-1636 cm-1 were assigned:1651 cm-1 belonged to the carbonyl stretching vibrations of flavonoids and chromones;1717 cm-1 belonged to tannins;1699 cm-1 belonged to carboxylic acids;1636 cm-1 belonged to anthraquinones and their derivatives.SD-IR spectroscopy further confirmed that the characteristicabsorption peaks at 1636,1618 and 1603 cm-1 could be used as markers that BXXTR-FU contained anthraquinones and their derivatives.Synchronous2 D-IR correlation spectra of chemical groups further confirmed the results of FT-IR and SD-IR spectroscopy.CONCLUSION:Our study strongly supported the necessity and importance of three-stage IR spectroscopy owing to its rapid and accurate identification of herbal formulations.
基金
Supported by National Science and Technology Major Projects for New Drug Development(2017ZX09305005)
Scientific Research Funds for High-Caliber Researchers of Shihezi University(RCSX201715)