摘要
OBJECTIVE:To develop the chromatographic fingerprint of Lonicera japonica(L.japonica)and evaluate the effects of polyploidy on the quality of L.japonica.METHODS:High-performance liquid chromatography(HPLC)methods used to establish the chromatographic fingerprint were developed.The quality of 11 batches of diploid L.japonica and 13 batches of tetraploid L.japonica collected from different regions across China were analyzed.The contents of five active compounds,consisting of chlorogenic acid,rutin,galuteolin,isochlorogenic acid A and quercetin,were further detected in L.japonica.RESULTS:The chromatographic fingerprint established by the optimized HPLC method was verified for qualitative analysis of L.japonica.Quantitative analysis showed that the contents of chlorogenic acid,isochlorogenic acid A,and quercetin in tetraploid plants were higher than those in diploid plants,whereas rutin and galuteolin contents in tetraploid plants were lower than those in diploid plants.CONCLUSION:The developed HPLC method is suitable for qualitative analysis of L.japonica.Polyploidy was indicated to influence the chemical properties of L.japonica.Tetraploid L.japonica shows potential for utilization as a medicinal plant with different active components.
OBJECTIVE: To develop the chromatographic fingerprint of Lonicera japonica(L. japonica) and evaluate the effects of polyploidy on the quality of L. japonica.METHODS: High-performance liquid chromatography(HPLC) methods used to establish the chromatographic fingerprint were developed. The quality of 11 batches of diploid L. japonica and 13 batches of tetraploid L. japonica collected from different regions across China were analyzed. The contents of five active compounds, consisting of chlorogenic acid, rutin, galuteolin, isochlorogenic acid A and quercetin, were further detected in L. japonica.RESULTS: The chromatographic fingerprint established by the optimized HPLC method was verified for qualitative analysis of L. japonica. Quantitative analysis showed that the contents of chlorogenic acid, isochlorogenic acid A, and quercetin in tetraploid plants were higher than those in diploid plants, whereas rutin and galuteolin contents in tetraploid plants were lower than those in diploid plants.CONCLUSION: The developed HPLC method is suitable for qualitative analysis of L. japonica. Polyploidy was indicated to influence the chemical properties of L. japonica. Tetraploid L. japonica shows potential for utilization as a medicinal plant with different active components.
基金
Supported by National Natural Science Foundation of China(Key Efficacy Components Screening and Their Action Mechanisms on Anti-influenza Virus Differences between Lonicerae Japonicae Flos and Lonicerae Flos,No.31370360)
the National Natural Science Foundation of Hainan(Cloning and Functional Characterization of Sg STOP1 in Stylosanthes,No.317268)
China Agriculture Research System(Haikou Green Manure Experimental Station,No.CARS-22-Z11).
