摘要
OBJECTIVE:To explore the protective mechanisms of the Traditional Chinese Medicine Bushenhuoxue(BSHX)in a rat model of vascular dementia(VD).METHODS:A rat model of VD was developed using bilateral common carotid artery occlusion(BCCAO).Rats were administered BSHX(10.14 or 5.07 g/kg),nimodipine(11.06 mg/kg;positive control),or saline(control)by gavage daily for 30 d post-surgery.Learning and memory abilities were assessed using the Morris water maze.Morphological changes in the hippocampus were observed using light microscopy(hematoxylin and eosin staining)and transmission electron microscopy(TEM).The m RNA and protein expression levels of brain-derived neurotrophic factor(BDNF),tyrosine receptor kinase B(Trk B),phosphatidyl inositol 3-kinase(PI3 K),serine/threonine kinase(AKT),and c AMP response element binding protein(CREB)were measured by real-time polymerase chain reaction(RT-PCR)and Western blot,respectively.RESULTS:Compared with the sham group,rats with BCCAO exhibited impaired learning and memory abilities(Morris water maze)and showed abnormalities in neuronal morphology(light microscopy)and ultrastructure(TEM)in the hippocampus.They also had decreased m RNA and protein expressions of BDNF,Trk B,PI3 K,AKT,and CREB in hippocampal tissue(all P<0.05).In rats with BCCAO,administration of BSHX attenuated deficits in learning and memory,improved the morphology and ultrastructure of hippocampal neurons,and enhanced m RNA and protein expression levels of BDNF,Trk B,PI3 K,AKT,and CREB(all P<0.05).CONCLUSION:BSHX may protect hippocampal neurons and improve learning and memory abilities,at least in part via the activation of BDNF/Trk B/PI3 K/AKT/CREB signaling.
OBJECTIVE: To explore the protective mechanisms of the Traditional Chinese Medicine Bushenhuoxue(BSHX) in a rat model of vascular dementia(VD).METHODS: A rat model of VD was developed using bilateral common carotid artery occlusion(BCCAO).Rats were administered BSHX(10.14 or 5.07 g/kg),nimodipine(11.06 mg/kg; positive control), or saline(control) by gavage daily for 30 d post-surgery.Learning and memory abilities were assessed using the Morris water maze. Morphological changes in the hippocampus were observed using light microscopy(hematoxylin and eosin staining) and transmission electron microscopy(TEM). The m RNA and protein expression levels of brain-derived neurotrophic factor(BDNF), tyrosine receptor kinase B(Trk B), phosphatidyl inositol 3-kinase(PI3 K), serine/threonine kinase(AKT), and c AMP response element binding protein(CREB) were measured by real-time polymerase chain reaction(RT-PCR) and Western blot, respectively.RESULTS: Compared with the sham group, rats with BCCAO exhibited impaired learning and memory abilities(Morris water maze) and showed abnormalities in neuronal morphology(light microscopy)and ultrastructure(TEM) in the hippocampus. They also had decreased m RNA and protein expressions of BDNF, Trk B, PI3 K, AKT, and CREB in hippocampal tissue(all P < 0.05). In rats with BCCAO, administration of BSHX attenuated deficits in learning and memory, improved the morphology and ultrastructure of hippocampal neurons, and enhanced m RNA and protein expression levels of BDNF, Trk B, PI3 K,AKT, and CREB(all P < 0.05).CONCLUSION: BSHX may protect hippocampal neurons and improve learning and memory abilities, at least in part via the activation of BDNF/Trk B/PI3 K/AKT/CREB signaling.
基金
Supported by Hebei Province Natural Science Fund(Protection Mechanism Research of Bushenhuoxue on Hippocampal Nerve in Rats with Vascular Dementia Based on BDNF/Trk B Signaling Pathway,No.H2015423057)
Hebei Provincial Department of Education Science and Technology Research Key Funding Project(Study on the Mechanism of Regulating the Autophagy and Apoptosis of Hippocampal Neurons in VD Rats by Bushenhuoxue Prescription,No.ZD2018009)
Research Capacity Enhancement Project of Hebei University of Chinese Medicine(Improvement of Hippocampal Synaptic Remodeling in Rats with Vascular Dementia by Regulating BDNF/Trk B Signaling Pathway Based on the"renal essence"theory,No.2019-11).
