胎盘植入性疾病中TGFB2 mRNA表达上调的临床意义

The clinical significance of upregulation of TGFB2 mRNA expression in placenta accreta spectrum disorders

目的探讨转化生长因子β2(TGFB2)mRNA在胎盘植入性疾病(PAS)中的临床意义。方法收集广西医科大学第一附属医院住院分娩的3例PAS患者胎盘组织,选取同期正常的5例非PAS患者胎盘组织作为对照组,对样本进行高通量测序,得到内部mRNA测序结果。之后从公共高通量数据库中筛选出3个相关数据集,先探讨各组之间TGFB2 mRNA的表达差异,再整合计算TGFB2 mRNA的标准均数差(SMD)和整合受试者操作特征(sROC)曲线,最后综合内部测序数据进一步分析TGFB2 mRNA在PAS与非PAS之间的表达差异。结果从公共高通量数据库筛选的3个数据集以及内部测序数据中TGFB2 mRNA表达水平相较于对照组均上调。整合计算3个数据集结果显示TGFB2 mRNA在PAS中表达显著上调(SMD=1.02,95%CI=0.02~2.02)。再结合本单位内部测序进一步综合分析证实,PAS中TGFB2 mRNA表达比非PAS样本显著上调(SMD=1.05,95%CI=0.47~1.64),sROC曲线下面积为0.87。结论高表达的TGFB2 mRNA可能促进PAS的发生发展,是潜在的生物标志物。

Objective To investigate the clinical significance of transforming growth factor beta 2(TGFB2)mRNA expression in placenta accreta spectrum(PAS)disorders.Methods The placental tissues were collected from 3 patients with PAS and 5 normal pregnant women in the same period who dilivered in the First Affiliated Hospital of Guangxi Medical University,and the samples were sequenced by high-throughput sequencing,and the internal mRNA sequencing results were obtained.3 TGFB2 expression datasets were screened from a public high-throughput database,and explored the expression differences of TGFB2 mRNA among the groups.Then,the standard mean deviation(SMD)of TGFB2 mRNA and the summary receiver operating characteristic(sROC)curve were integrated and calculated.Finally,internal sequencing data was integrated to further analyze the expression differences of TGFB2 mRNA between PAS and normal placenta tissue.Results The expression levels of TGFB2 mRNA in the three datasets screened from public high-throughput databases and internal sequencing data were upregulated compared to the control group.The results of integrating three datasets showed that TGFB2 mRNA was significantly upregulated in PAS(SMD=1.02,95%CI=0.02-2.02).The results of the in-house sequencing confirmed that the expression of TGFB2 mRNA in PAS was significantly upregulated compared to normal placenta tissue(SMD=1.05,95%CI=0.47-1.64),with an area under the sROC curve of 0.87.Conclusion Highly expressed TGFB2 may promote the occurrence of PAS,which is a potential biomarker.