短筒兔耳草化学成分研究

Chemical Constituents of Lagotis brevituba

目的:研究短筒兔耳草Lagotis brevituba Maxim.的化学成分。方法:短筒兔耳草70%乙醇提取物,采用大孔树脂、MCI、ODS、硅胶、Sephadex LH-20葡聚糖凝胶、半制备高效液相色谱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果:从短筒兔耳草中分离鉴定得到14个化合物,分别为:大车前苷(1)、松果菊苷(2)、毛蕊花糖苷(3)、车前草苷D(4)、chionoside J(5)、maxoside(6)、turrilliside A(7)、juniperoside(8)、lagotisoside A(9)、土荆皮苷C(10)、4-羟基苯甲酸-β-D-葡萄糖酯(11)、木犀草素-7-O-槐糖苷(12)、6-羟基木犀草素-7-O-葡萄糖苷(13)、对羟基苯甲酸(14)。结论:其中,化合物5~8、10~13为首次从兔耳草属植物中分离得到,化合物4、9为首次从该植物中分离得到。

Objective:To study the chemical constituents of Lagotis brevituba.Methods:Using macroporous resin,MCI,ODS,silica gel,Sephadex LH-20,semi-prepared HPLC and other methods were used to isolated and purified the compounds from 70%ethanol extract of Lagotis brevituba.The structures of the compounds were identified according to the physical and chemical properties and spectral data.Results:Fourteen compounds were isolated from Lagotis brevituba and identified as plantamajoside(1),echinacoside(2),acteoside(3),plantainoside D(4),chionoside J(5),maxoside(6),turrilliside A(7),juniperoside(8),lagotisoside A(9),pseudolaroside C(10),4-hydroxybenzoic acid-β-D-glucose ester(11),luteolin-7-O-sophoroside(12),6-hydroxyluteolin-7-O-glucoside(13),4-hydroxybenzoic acid(14),respectively.Conclusion:Among them,compounds 5 to 8 and 10 to 13 are isolated from Lagotis genus for the first time,compounds 4 and 9 are isolated from Lagotis brevituba for the first time.