番茄红素调控PI3K/AKT通路抑制卵巢癌细胞上皮间质转化的作用及机制

Effect and Mechanism of Lycopene on Inhibiting Epithelial Mesenchymal Transformation of Ovarian Cancer Cells by Regulating PI3K/AKT Pathway

目的:探讨番茄红素通过调控PI3K/AKT通路抑制卵巢癌细胞上皮间质转化(EMT)的作用及机制。方法:培养卵巢癌细胞株SKOV3,实验设空白对照组、番茄红素(5、10、20μg/L)组、番茄红素(20μg/L)+IGF-1(100μg/L)组、番茄红素(20μg/L)+PBS组。检测并比较各组细胞存活率、迁移能力及E-cadherin、N-cadherin、Vimentin、p-PI3K、p-AKT表达。结果:与空白对照组比较,番茄红素各浓度组细胞存活率、迁移能力显著降低,E-cadherin mRNA及蛋白表达显著升高,N-cadherin、Vimentin mRNA及蛋白表达显著降低,p-PI3K、p-AKT蛋白表达显著降低(P<0.05)。PI3K/AKT信号通路激动剂IGF-1可逆转番茄红素对SKOV3细胞的作用。结论:番茄红素对卵巢癌SKOV3细胞的存活、迁移及EMT具有抑制作用,其机制可能与抑制PI3K/AKT信号通路有关。

Objective:To investigate the effect and mechanism of lycopene on inhibiting epithelial mesenchymal transformation(EMT)of ovarian cancer cells by regulating PI3K/AKT pathway.Methods:Ovarian cancer cell line SKOV3 was cultured,the experiment included normal control group,lycopene(5,10,20μg/L)groups,lycopene(20μg/L)with IGF-1(100μg/L)group,lycopene(20μg/L)with PBS group.The cell survival rate,migration ability and the expressions of E-cadherin,N-cadherin,Vimentin,p-PI3K,p-AKT in each group were detected and compared.Results:Compared with the normal control group,the cell survival rate and migration ability in each lycopene group were significantly decreased,the mRNA and protein expression of E-cadherin was significantly increased,the mRNA and protein expressions of N-cadherin,Vimentin were significantly decreased,the protein expressions of p-PI3K,p-AKT were significantly decreased(P<0.05).PI3K/AKT signaling pathway agonist IGF-1 reversed the effects of lycopene on SKOV3 cells.Conclusion:Lycopene inhibits the survival,migration and EMT of ovarian cancer SKOV3 cells,the mechanism may be related to the inhibition of PI3K/AKT signaling pathway.