葛根化学成分研究

Chemical Constituents of Pueraria lobata Roots

目的:研究葛根的化学成分。方法:采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、制备高效液相色谱等方法进行分离纯化,通过理化性质及波谱数据分析鉴定结构。结果:从葛根中分离得到37个化合物,分别鉴定为:丁香酸(1)、丁香醛(2)、对羟基苯甲酸乙酯(3)、(-)-鹰嘴豆醇(4)、甘草素(5)、刺芒柄花素(6)、3′-羟基-大豆苷元(7)、染料木素(8)、毛蕊异黄酮(9)、印度黄檀苷(10)、染料木苷(11)、葛根素(12)、芒柄花苷(13)、6″-O-乙酰基大豆苷(14)、大豆苷(15)、葛根素-4′-O-葡萄糖苷(16)、6″-巴豆酰大豆苷(17)、asphodelin A(18)、8-C-β-D-glucopyranosyl-4′,7-dihydroxy-3′-methoxyisoflavone-4′-O-β-D-glucopyranoside(19)、澳白檀苷(20)、大豆苷元-8-C-芹菜糖-(1→6)-葡萄糖苷(21)、染料木素-7-O-β-D-呋喃芹糖基-(1→6)-O-β-D-吡喃葡萄糖苷(22)、毛蕊异黄酮-7-O-β-D-葡萄糖苷(23)、7,2′,4′-三羟基异黄酮(24)、liquiritigenin-7-methyl ether(25)、葛酚苷元B(26)、葛酚苷元A(27)、kuzubutenolide A(28)、葛酚苷B(29)、葛根素-6″-O-木糖苷(30)、3′-羟基葛根素(31)、formononetin-8-C-[β-D-apiofuranosyl-(1→6)]-β-D-glucopyranoside(32)、formononetin-8-C-[β-D-xylopyranosyl-(1→6)]-β-D-glucopyranoside(33)、芒柄花素-7-O-β-D-呋喃芹糖基-(1→6)-β-D-吡喃葡萄糖苷(34)、3′-甲氧基葛根素(35)、大豆苷元(36)、3-(3,4-dimethoxy-2-hydroxyphenyl)-7-hydroxycoumarin(37)。结论:其中,化合物1、18、20、23和37为首次从葛属植物中分离得到,化合物17和22为首次从该植物中分离得到。

Objective:To study the chemical constituents from the roots of Pueraria lobata.Methods:The chemical constituents were isolated and purified by silica gel column chromatography,Sephadex LH-20 column chromatography and HPLC.The structures were identified by spectral data and physicochemical properties analysis.Results:Thirty-seven compounds were isolated from the roots of Pueraria lobata and identified as syringic acid(1),syringaldehyde(2),ethyl p-hydroxybenzoate(3),(-)-garbanzol(4),liquiritigenin(5),formononetin(6),3'-hydroxy-daidzein(7),genistein(8),calycosin(9),sissotrin(10),genistin(11),puerarin(12),ononin(13),6″-O-acetyl-daidzin(14),daidzin(15),puerarin-4'-O-glucoside(16),6″-crotonyldaidzin(17),asphodelin A(18),8-C-β-D-glucopyranosyl-4',7-dihydroxy-3'-methoxyisoflavone-4'-O-β-D-glucopyranoside(19),lanceolarin(20),daidzein-8-C-apiosyl-(1→6)-glucoside(21),ambocin(22),calycosin-7-O-β-D-glucoside(23),7,2',4'-trihydroxyisoflavone(24),liquiritigenin-7-methyl ether(25),puerol B(26),puerol A(27),kuzubutenolide A(28),pueroside B(29),puerarin-6″-O-xyloside(30),3'-hydroxypuerarin(31),formononetin-8-C-[β-D-apiofuranosyl-(1→6)]-β-D-glucopyranoside(32),formononetin-8-C-[β-D-xylopyranosyl-(1→6)]-β-D-glucopyranoside(33),formononetin-7-O-β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside(34),3'-methoxypuerarin(35),daidzein(36),3-(3,4-dimethoxy-2-hydroxyphenyl)-7-hydroxycoumarin(37),respectively.Conclusion:Among them,compounds 1,18,20,23,37 are isolated from Pueraria genus for the first time,compounds 17,22 are isolated from Pueraria lobata for the first time.