黄帚橐吾乙酸乙酯部位化学成分研究

Chemical Constituents from Ethyl Acetate Extract of Ligularia virgaurea

目的:研究黄帚橐吾Ligularia virgaurea(Maxim.)Mactf.干燥全草的化学成分。方法:黄帚橐吾用75%乙醇提取,分别用石油醚、乙酸乙酯萃取,对乙酸乙酯部位采用硅胶、凝胶、高效液相色谱等方法及各种波谱数据(MS、^(1)H-NMR、^(13)C-NMR)进行分离鉴定。结果:从黄帚橐吾乙酸乙酯部位中分离得到12个化合物,分别鉴定为:toluccanolide B(1)、6β-(2′-hydroxymethylacryloyloxy)-1(10)-en-9-oxofuranoeremophilane(2)、6β-(2′-hydroxymethylacryloyloxy)-9-oxofuranoeremophilane(3)、6-(2′-hydroxymethylacryloyloxy)-7,8-epoxyeremophil-1(10)-en-(12,8)-olide(4)、epicacalone(5)、cacalone(6)、secovirgaurenol-6-O-(2′-hydroxymethyl)acrylate(7)、6β-(2′-hydroxymethylacryloyloxy)-fukinospirolide A(8)、oplodiol(9)、4-(3′-hydroxypropyl)-2,6-dimethoxyphenol-3′-O-β-D-glcoside(10)、丁香苷(11)、果糖(12)。结论:其中,化合物2~4、7~12为首次从黄帚橐吾中分离得到。

Objective:To study the chemical constituents of Ligularia virgaurea whole herb.Methods:The whole herbs of Ligularia virgaurea were extracted with 75%ethanol,petroleum ether and ethyl acetate,respectively,the compounds of ethyl acetate extract were isolated by column chromatography,such as silica gel,gel chromatography,HPLC and identified by various spectral data(MS,^(1)H-NMR,^(13)C-NMR).Results:Twelve compounds were isolated from ethyl acetate extract of Ligularia virgaurea and identified as toluccanolide B(1),6β-(2′-hydroxymethylacryloyloxy)-1(10)-en-9-oxofuranoeremophilane(2),6β-(2′-hydroxymethylacryloyloxy)-9-oxofuranoeremophilane(3),6-(2′-hydroxymethylacryloyloxy)-7,8-epoxyeremophil-1(10)-en-(12,8)-olide(4),epicacalone(5),cacalone(6),secovirgaurenol-6-O-(2′-hydroxymethyl)acrylate(7),6β-(2′-hydroxymethylacryloyloxy)-fukinospirolide A(8),oplodiol(9),4-(3′-hydroxypropyl)-2,6-dimethoxyphenol-3′-O-β-D-glcoside(10),syringin(11),fructose(12),respectively.Conclusion:Among them,compounds 2 to 4,7 to 12 are isolated from Ligularia virgaurea for the first time.