摘要
目的:建立临床重用90 g与小剂量30 g久煎的黄芪水煎液指纹图谱,并进行一致性评价研究。方法:采用HPLC-ELSD法建立黄芪90 g提取50 min和30 g提取6个不同时间(50、100、150、200、250、300 min)的水煎液指纹图谱;利用聚类分析(HCA)和偏最小二乘判别分析(PLS-DA)对共有峰峰面积进行分析,并进行黄芪甲苷的含量测定,研究化学成分变化情况。结果:70批黄芪水煎液HPLC-ELSD指纹图谱中有6个共有峰,指认了其中3个成分,分别为黄芪甲苷、黄芪皂苷Ⅱ、黄芪皂苷Ⅰ,HPLC-ELSD指纹图谱相似度为0.758~0.978。聚类分析与偏最小二乘判别分析结果一致,黄芪重用90 g提取50 min和黄芪30 g提取300 min聚为一类。结论:黄芪小剂量30 g提取300 min时可与重用90 g提取50 min水煎液指纹图谱较一致,临床使用中单味黄芪可适当延长提取时间,复方中黄芪或可先煎、久煎代替重用。
Objective:To establish the fingerprints of the decoction of Astragali Radix with clinical reuse of 90 g and a small dose of 30 g for a long time,and to evaluate the consistency.Methods:HPLC-ELSD method was used to establish the fingerprints of the decoction of Astragali Radix extracted at 90 g for 50 min and 30 g for 6 different extraction times,including 50,100,150,200,250,300 min.Cluster analysis(HCA)and partial least square discriminant analysis(PLS-DA)were used to analyze the common peak areas,and the content of astragalosideⅣwas determined to study the chemical composition changes.Results:There were 6 common peaks in the HPLC-ELSD fingerprints of the 70 batches of Astragali Radix water decoction,and 3 of them were identified as astragalosideⅣ,astragalosideⅡand astragalosideⅠ,respectively.The similarity of HPLC-ELSD fingerprints ranged from 0.758 to 0.978.The results of cluster analysis and partial least squares discriminant analysis were consistent.The Astragali Radix which was reused for 90 g extraction for 50 min and Astragali Radix which was extracted for 30 g extraction for 300 min were clustered into one class.Conclusion:The fingerprint of Astragali Radix extracted at a small dose of 30 g for 300 min is consistent with the fingerprint of water decoction extracted at 90 g for 50 min.In clinical use,the extraction time of single Astragali Radix can be appropriately prolonged,and the Astragali Radix in compound may be replaced by decocting first or long decocting.
作者
韦花花
常丽静
李明月
邱智东
陈新
WEI Hua-hua;CHANG Li-jing;LI Ming-yue;QIU Zhi-dong;CHEN Xin(Key Laboratory for Effective Substances of Genuine Medicinal Material in Changbai Mountain,Changchun University of Chinese Medicine,Changchun 130117,China)
出处
《中药材》
CAS
北大核心
2022年第3期652-658,共7页
Journal of Chinese Medicinal Materials
基金
校企合作项目(2018-2200-02-000490)
吉林省科技发展计划项目(20180201089YY)
重大疫情防治中药方剂储备库建设项目(吉中医药发[2021]11号)
关键词
黄芪
重用
久煎
指纹图谱
化学计量学
黄芪甲苷
Astragali Radix
Reuse
Long decoction
Fingerprint
Stoichiometry
AstragalosideⅣ