补肾调冲汤对薄型子宫内膜大鼠细胞焦亡的作用研究

Effect of Bushen Tiaochong Decoction on Pyroptosis in Rats with Thin Endometrium

目的:探讨补肾调冲汤对薄型子宫内膜大鼠细胞焦亡的作用。方法:选取动情周期正常、未交配的SPF级雌性SD大鼠60只,按随机数字表法随机分出10只作为正常对照组,剩余50只建立大鼠薄型子宫内膜模型并分为模型组、戊酸雌二醇片(0.1 mg/kg)组及补肾调冲汤低(8.525 g/kg)、中(17.05 g/kg)、高(34.10 g/kg)剂量组,每组各10只,灌胃给药。正常对照组、模型组均予等体积生理盐水灌胃。1次/d,连续15 d。取各组大鼠子宫组织HE染色,显微镜下测量子宫内膜厚度及观察子宫内膜组织病理学变化,免疫组化法检测子宫内膜CD68巨噬细胞数目;RT-PCR法、Western blot法检测子宫内膜Caspase-1、IL-1β、IL-18、GSDMD mRNA及蛋白表达。结果:与模型组比较,补肾调冲可使模型大鼠子宫内膜厚度、子宫内膜腺体、血管均不同程度增加,组织纤维化不同程度地减少,并能减少薄型子宫大鼠子宫内膜间质CD68巨噬细胞数目及Caspase-1、IL-1β、IL-18、GSDMD mRNA及蛋白表达。结论:补肾调冲汤能增加薄型子宫内膜大鼠子宫内膜厚度,改善子宫内膜组织形态结构,减少薄型子宫内膜大鼠CD68巨噬细胞数目,下调细胞焦亡相关因子Caspase-1、IL-1β、IL-18、GSDMD mRNA及蛋白的表达,其作用机制可能与减轻损伤子宫内膜炎症反应,在一定程度上抑制细胞焦亡作用,促进受损子宫内膜修复和血管生成有关。

Objective:To study the effect of Bushen tiaochong decoction on the pyroptosis of thin endometrial rats.Methods:Sixty non-mating female SPF SD rats with normal estrous cycle were selected,and 10 rats were randomly divided into normal control group according to random number table method.The remaining 50 rats were used to establish the thin endometrium model and divided into model group,estradiol valerate tablet(0.1 mg/kg)group,Bushen tiaochong decoction low(8.525 g/kg),medium(17.05 g/kg)and high(34.10 g/kg)dose groups,with 10 rats in each group,thses rats were given the drug by gavage.Normal control group and model group were given equal volume of normal saline intragastric administration,once per day for 15 days.The endometrial tissues of each group were stained with HE,the thickness of endometrial was measured under microscope,and the histopathological changes of endometrium were observed.The number of macrophages CD68 in endometrium was detected by immunohistochemistry.The mRNA and protein expressions of Caspase-1,IL-1β,IL-18 and GSDMD in endometrium were detected by RT-PCR and Western blot.Results:Compared with model group,endometrial thickness,endometrial glands and blood vessels of model rats were increased after treated with Bushen tiaochong decoction,tissue fibrosis was reduced to varying degrees,and the number of CD68 macrophages in the endometrial stromal of rats with thin uterus,and the expressions of Caspase-1,IL-1β,IL-18,GSDMD mRNA and proteins were significantly decreased.Conclusion:Bushen tiaochong decoction can increase the thickness of thin endometrium rats,improve the morphological structure of endometrium,reduce the number of CD68 macrophages,down-regulate the expressions of Caspase-1,IL-1β,IL-18 and GSDMD mRNA and proteins related to cell coke death in rats with thin endometrium.The mechanism of action may be related to the alleviating of inflammatory response of damaged endometrium,inhibiting cell coke death and promoting repair and angiogenesis of damaged endometrium to a certain extent.