摘要
目的:建立UHPLC-QTRAP-MS/MS法同时测定尖叶假龙胆中芒果苷、异荭草素、去甲当药醇苷、异牡荆素、当药醇苷、去甲基雏菊叶龙胆酮、雏菊叶龙胆酮7个成分含量。方法:采用Shim-pack GIST C_(18)(100 mm×2.1 mm,2μm)色谱柱;以乙腈-0.1%甲酸溶液为流动相,梯度洗脱;流速为0.3 mL/min;柱温为35℃;采用电喷雾离子源(ESI),多反应离子监测(MRM)模式进行负离子监测。结果:上述7个成分之间分离度良好,且分别在一定的浓度范围内与峰面积的线性关系良好(R^(2)≥0.9990),平均加样回收率为98.76%~102.74%,RSD<2.50%,方法重复性、仪器精密度和供试品溶液的稳定性均良好。结论:该方法操作简便,快速,灵敏度高,可用于尖叶假龙胆中成分的含量测定,为尖叶假龙胆药材的质量控制提供了新方法。
Objective:To establish an UHPLC-QTRAP-MS/MS method for the simultaneous content determination of mangiferin,isoorientin,norswertianolin,isovitexin,swertianolin,demethylbellidifolin,bellidifolin in Gentianella acuta whole herb.Methods:The chromatographic column was Shim-pack GIST C_(18)(100 mm×2.1 mm,2μm)column,the mobile phase was acetonitrile and 0.1%formic acid solution with gradient elution,flow rate was 0.3 mL/min,column temperature was 35℃.Electrospray ion source(ESI)and multi-reactive ion monitoring(MRM)were used to monitor negative ions.Results:The separation of the seven components was good,and the linear relationship with the peak areas was good in a certain concentration ranges(R^(2)≥0.9990).The average recoveries were 98.76%to 102.74%,RSD<2.50%.The method repeatability,instrument precision and stability of the tested solution were good.Conclusion:The method is simple,rapid and sensitive,and can be used for the determination of components in Gentianella acuta,which provides a new method for the quality control of Gentianella acuta.
作者
张闯
彭小园
刘兴超
周伟伟
郭亚净
杨洪霞
李爱英
ZHANG Chuang;PENG Xiao-yuan;LIU Xing-chao;ZHOU Wei-wei;GUO Ya-jing;YANG Hong-xia;LI Ai-ying(Hebei University of Chinese Medicine,Shijiazhuang 050200,China;Hebei Key Laboratory of Chinese Medicine Research on Cardio-cerebrovascular Disease/Hebei Higher Education Institute Applied Technology Research Center on TCM Formula Preparation,Shijiazhuang 050091,China)
出处
《中药材》
CAS
北大核心
2021年第9期2127-2131,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金面上项目(81573698)
河北省重点研发计划项目(20372501D)
河北省中医药管理局资助项目(2020139)
河北省省属高校基本科研业务费青年教师科研提升项目(YJZ2019001)
河北省教育厅在读研究生创新能力培养资助项目(CXZZBS2020147)