UHPLC-QTRAP-MS/MS法同时测定尖叶假龙胆中7个成分的含量

Simultaneous Determination of Seven Components in Gentianella acuta Whole Herb by UHPLC-QTRAP-MS/MS

目的:建立UHPLC-QTRAP-MS/MS法同时测定尖叶假龙胆中芒果苷、异荭草素、去甲当药醇苷、异牡荆素、当药醇苷、去甲基雏菊叶龙胆酮、雏菊叶龙胆酮7个成分含量。方法:采用Shim-pack GIST C_(18)(100 mm×2.1 mm,2μm)色谱柱;以乙腈-0.1%甲酸溶液为流动相,梯度洗脱;流速为0.3 mL/min;柱温为35℃;采用电喷雾离子源(ESI),多反应离子监测(MRM)模式进行负离子监测。结果:上述7个成分之间分离度良好,且分别在一定的浓度范围内与峰面积的线性关系良好(R^(2)≥0.9990),平均加样回收率为98.76%~102.74%,RSD<2.50%,方法重复性、仪器精密度和供试品溶液的稳定性均良好。结论:该方法操作简便,快速,灵敏度高,可用于尖叶假龙胆中成分的含量测定,为尖叶假龙胆药材的质量控制提供了新方法。

Objective:To establish an UHPLC-QTRAP-MS/MS method for the simultaneous content determination of mangiferin,isoorientin,norswertianolin,isovitexin,swertianolin,demethylbellidifolin,bellidifolin in Gentianella acuta whole herb.Methods:The chromatographic column was Shim-pack GIST C_(18)(100 mm×2.1 mm,2μm)column,the mobile phase was acetonitrile and 0.1%formic acid solution with gradient elution,flow rate was 0.3 mL/min,column temperature was 35℃.Electrospray ion source(ESI)and multi-reactive ion monitoring(MRM)were used to monitor negative ions.Results:The separation of the seven components was good,and the linear relationship with the peak areas was good in a certain concentration ranges(R^(2)≥0.9990).The average recoveries were 98.76%to 102.74%,RSD<2.50%.The method repeatability,instrument precision and stability of the tested solution were good.Conclusion:The method is simple,rapid and sensitive,and can be used for the determination of components in Gentianella acuta,which provides a new method for the quality control of Gentianella acuta.