摘要
目的:优化蜘蛛香的超声提取工艺。方法:采用一测多评法测定蜘蛛香中新绿原酸、绿原酸、咖啡酸、异绿原酸B、橙皮苷、异绿原酸A、异绿原酸C、乙酰缬草三酯和缬草三酯的含量,以乙醇浓度、液料比、提取时间为考察因素,用熵权法赋权计算蜘蛛香中9种成分提取量综合评分为评价指标,通过Box-Behnken响应面法优选蜘蛛香提取工艺。结果:优化得到的蜘蛛香提取工艺为:药材加56倍量的66%乙醇,超声(200 W,35 kHz)提取34 min。验证试验表明各指标成分提取量及综合评分RSD均小于3%。结论:该提取工艺稳定、可行,有效成分提取率较高,适用于蜘蛛香的提取。
Objective:To optimize of ultrasonic extraction technology of Valeriana jatamansi rhizomes and roots.Methods:The contents of new chlorogenic acid,chlorogenic acid,caffeine,isochlorogenic acid B,hesperidin,isochlorogenic acid A,isochlorogenic acid C,acevaltratum and valepotriate in Valeriana jatamansi rhizomes and roots were quantitative analysis by QAMS method.Using the ethanol concentration,liquid-material ratio and extraction time as investigation factors,the entropy weight method was used to calculate the comprehensive scores of 9 components contents in Valeriana jatamansi rhizomes and roots,the Box-Behnken response surface methodology was adopted to optimize the extraction process of Valeriana jatamansi rhizomes and roots.Results:The optimum extraction process was that the medicinal materials were extracted by ultrasound(200 W,35 kHz)for 34 min with 56 times amount of 66%ethanol.The results of verification test showed that RSD of the contents of components and the comprehensive scores of each index was less than 3%.Conclusion:The extraction process is stable and feasible,and the extraction rate of effective components is high.It is suitable for the extraction of Valeriana jatamansi rhizomes and roots.
作者
付洋
程盛勇
陈慧
苑天红
张玥
杨军
罗喜荣
FU Yang;CHENG Sheng-yong;CHEN Hui;YUAN Tian-hong;ZHANG Yue;YANG Jun;LUO Xi-rong(College of Pharmacy,Guizhou Medical University,Guiyang 550025,China;College of Basic Medicine,Guizhou Medical University,Guiyang 550025,China;Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550081,China)
出处
《中药材》
CAS
北大核心
2021年第2期403-407,共5页
Journal of Chinese Medicinal Materials
基金
贵州省联合基金项目(黔科合LH字[2014]7091号)
贵州省社发攻关项目(黔科合SY字[2015]3032号)
贵州省中药现代化专项项目(黔科合ZY字[2012]3010号)
关键词
蜘蛛香
响应面法
熵权法
多指标
超声提取
一测多评法
Rhizomes and roots of Valeriana jatamansi Jones
Response surface method
Entropy weight method
Multiple indexes
Ultrasonic extraction
QAMS