响应面法结合熵权法多指标优选蜘蛛香提取工艺

Study on the Optimization of the Extraction Technology of Valeriana jatamansi Rhizomes and Roots Based on Response Surface Method Combined with Entropy Weight Method

目的:优化蜘蛛香的超声提取工艺。方法:采用一测多评法测定蜘蛛香中新绿原酸、绿原酸、咖啡酸、异绿原酸B、橙皮苷、异绿原酸A、异绿原酸C、乙酰缬草三酯和缬草三酯的含量,以乙醇浓度、液料比、提取时间为考察因素,用熵权法赋权计算蜘蛛香中9种成分提取量综合评分为评价指标,通过Box-Behnken响应面法优选蜘蛛香提取工艺。结果:优化得到的蜘蛛香提取工艺为:药材加56倍量的66%乙醇,超声(200 W,35 kHz)提取34 min。验证试验表明各指标成分提取量及综合评分RSD均小于3%。结论:该提取工艺稳定、可行,有效成分提取率较高,适用于蜘蛛香的提取。

Objective:To optimize of ultrasonic extraction technology of Valeriana jatamansi rhizomes and roots.Methods:The contents of new chlorogenic acid,chlorogenic acid,caffeine,isochlorogenic acid B,hesperidin,isochlorogenic acid A,isochlorogenic acid C,acevaltratum and valepotriate in Valeriana jatamansi rhizomes and roots were quantitative analysis by QAMS method.Using the ethanol concentration,liquid-material ratio and extraction time as investigation factors,the entropy weight method was used to calculate the comprehensive scores of 9 components contents in Valeriana jatamansi rhizomes and roots,the Box-Behnken response surface methodology was adopted to optimize the extraction process of Valeriana jatamansi rhizomes and roots.Results:The optimum extraction process was that the medicinal materials were extracted by ultrasound(200 W,35 kHz)for 34 min with 56 times amount of 66%ethanol.The results of verification test showed that RSD of the contents of components and the comprehensive scores of each index was less than 3%.Conclusion:The extraction process is stable and feasible,and the extraction rate of effective components is high.It is suitable for the extraction of Valeriana jatamansi rhizomes and roots.