宣白承气汤合桑贝散治疗疫毒闭肺型新型冠状病毒肺炎(COVID-19)的机理研究

Mechanism of Xuanbai Chengqi Decoction and Sangbei Power in Treating Epidemic-closed Lung Type COVID-19

目的:基于网络药理学探讨宣白承气汤合桑贝散治疗疫毒闭肺型新型冠状病毒肺炎的机理。方法:通过TCMSP筛选和预测宣白承气汤合桑贝散可能的入血活性成分和作用基因靶点;下载血管紧张素转化酶2(Angiotensin converting enzyme 2,ACE2)相关基因靶点;采用STRING数据库绘制成分-靶点的PPI网络;提取宣白承气汤合桑贝散主要作用的靶点基因与ACE2相关基因的交集;采用DAVID数据库分析筛选宣白承气汤合桑贝散作用关键靶点与机制。结果:在TCMSP数据库共检索出与宣白承气汤合桑贝散方相关的活性成分496个,根据药代动力学参数筛选获得78个入血活性成分,并检索出与这些成分相关的靶点761个;下载得到ACE2相关的基因5556个;中药成分靶点和ACE2相关基因靶点取交集后得到49个关键基因;宣白承气汤合桑贝散作用的基因主要涉及细胞酮代谢、细胞内蛋白质转运、内肽酶抑制剂活性等,主要与Jak-STAT信号通路、产生IgA的肠道免疫网络途径、补体和凝血级联途径等相关。结论:宣白承气汤合桑贝散能通过49个基因位点作用于ACE2,其机制与细胞酮代谢、抑制蛋白质进入细胞内等相关。

Objective:To explore the mechanism of Xuanbai chengqi decoction and Sangbei power in the treatment of epidemic-closed lung type COVID-19 by network pharmacology.Methods:The potential blood active components and gene targets of Xuanbai chengqi decoction and Sangbei power were screened and predicted by TCMSP;The angiotensin converting enzyme 2(ACE2)related gene targets were downloaded;The PPI network of components-targets was plotted by STRING database.The intersection of ACE2-related genes and target genes of Xuanbai chengqi decoction and Sangbei power was extracted;The DAVID database was used to analyze and screen the key targets and mechanisms of Xuanbai chengqi decoction and Sangbei power.Results:A total of 496 active ingredients related to Xuanbai chengqi decoction and Sangbei power were retrieved from TCMSP database.According to the pharmacokinetic parameters,78 active components in blood were screened and 761 targets were retrieved.5556 ACE2-related genes were downloaded.49 key genes were obtained after the intersection of Chinese medicine component targets and ACE2 related gene targets;The genes affected by Xuanbai chengqi decoction and Sangbei power were mainly involved in cytoketone metabolism,intracellular protein transport,internal peptidase inhibitor activity and others,which were mainly related to the signaling pathway of the Jak-STAT,the intestinal immune network pathway of producing IgA,complement and coagulation cascade pathway,etc.Conclusion:Xuanbai chengqi decoction and Sangbei power can act on ACE2 through 49 gene loci,and its mechanism is related to cellular ketone metabolism and inhibition of protein entry into cells.