红景天苷通过调节Nrf2/HO-1和PPARγ/CEBPα信号通路抑制高脂诱导的大鼠肥胖

Inhibition Effect of Salidroside on High Fat Induced Obesity Rats by Regulating Nrf2/HO-1 and PPARγ/CEBPα Signaling Pathways

目的:观察红景天苷对高脂饲料诱导大鼠肥胖的治疗作用,并探讨其可能机制。方法:按文献方法建立肥胖模型,实验分为正常对照组、模型组及红景天苷50、100 mg/kg组;给药组灌胃给予相应的药物,1次/d,连续4 w。每周称量1次体质量和计算1次饲料消耗量和利用率。末次给药12 h后取血,进行Glu、INS、TG、TC、HDL-C、LDL-C含量检测和胰岛功能HOMA-IR、HOMA-β计算,取腹部和附睾脂肪组织计算质量指数;检测附睾脂肪组织TAC、GSH-Px、SOD活性和MDA含量并进行形态学分析;并检测附睾脂肪组织中Nrf2、HO-1、PPARγ、CEBPαmRNA表达。结果:红景天苷(50、100 mg/kg)能显著降低高脂诱导肥胖大鼠体质量、饲料利用率及血清Glu、INS、TG、TC、LDL-C水平并能降低HOMA-IR及附睾脂肪组织中MDA含量;升高血清HDL-C含量和HOMA-β及附睾脂肪组织中TAC、SOD、GSH-Px活性;降低腹部和附睾脂肪质量指数及附睾脂肪细胞大小和面积;升高附睾脂肪组织中Nrf2、HO-1 mRNA表达并降低脂肪组织中PPARγ、CEBPαmRNA表达。结论:红景天苷对高脂诱导大鼠肥胖具有较好的治疗作用,其作用机制与调节Nrf2/HO-1和PPARγ/CEBPα信号通路,增强抗氧化系统功能和抑制血糖、血脂升高有关。

Objective:To observe the therapeutic effect of salidroside on obesity rats induced by high-fat diet,to explore its possible mechanism.Methods:The obesity model was established according to the literature method,the rats were randomly divided into normal control group,model group,salidroside groups(50,100 mg/kg);The administration groups were given the corresponding medicine by gavage,once a day for 4 weeks,weighing the mass once a week and calculating the feed consumption and utilization rate once a week.The blood was taken 12 hours after the last administration,the contents of Glu,INS,TG,TC,HDL-C and LDL-C were detected and HOMA-IR and HOMA-βof islet function were calculated.The weight indexes of abdominal and epididymal adipose tissues were calculated.TAC,GSH-Px,SOD activities and MDA content in adipose tissue of epididymis were detected and morphological analysis was carried out.The expressions of Nrf2,HO-1,PPARγand CEBPαmRNA in adipose tissue of epididymis were detected.Results:Salidroside(50,100 mg/kg)could significantly reduce the body weight,feed utilization rate,Glu,INS,TG,TC,LDL-C levels in serum,HOMA-IR and MDA contents in epididymal adipose tissue of obesity rats induced by high-fat diet,increase HDL-C content in serum and HOMA-βand TAC,SOD,GSH-Px activities in epididymal adipose tissue,decrease the fat mass indexes of abdomen and epididymis and the size and area of epididymal adipocytes,increase the expressions of Nrf2 and HO-1 mRNA in epididymal adipose tissue and decrease the expressions of PPARγand CEBPαmRNA in adipose tissue.Conclusion:Salidroside has a good therapeutic effect on high fat induced obesity rats,its mechanism is related to regulating Nrf2/HO-1 and PPARγ/CEBPαsignaling pathways,enhancing antioxidant system function and inhibiting blood glucose and lipid elevation.