龙脷叶化学成分研究

Chemical Constituents from Sauropus spatulifolius

目的:研究龙脷叶的化学成分。方法:采用AB-8大孔吸附树脂、硅胶、ODS、Sephadex LH-20、高效液相制备色谱等方法进行分离纯化,采用1H-NMR、13C-NMR等波谱技术对分离得到的化合物进行结构鉴定。结果:从龙脷叶中分离得到了16个化合物,分别鉴定为:2,4-二叔丁基苯酚(1)、3,5-二叔丁基-4-羟基苯丙酸己酯(2)、对羟基苯甲酸(3)、N-羟乙基-2-乙酰基吡咯(4)、3-甲氧基-4-羟基苯甲酸(5)、咖啡酸(6)、(-)-lyoniresinol-3α-O-β-D-glucopyranoside(7)、对羟基肉桂酸(8)、3-甲氧基-4-羟基肉桂酸(9)、7-megastigmene-3,6,9-triol(10)、7-megastigmane-3,9,10-triol(11)、N-(3-carboxypropyl)-2-acetylpyrrole(12)、槲皮素-3-O-β-D-葡萄糖苷(13)、3-O-β-glucosyl-(1→6)-β-D-glucosyl-quercetin(14)、山柰酚-3-O-β-D-葡萄糖苷(15)、3-O-β-glucosyl-(1→6)-β-D-glucosyl-kaempferol(16)。结论:其中,化合物2、3、5、10、11、15为首次从该属植物中分离得到。

Objective:To study the chemical constituents from Sauropus spatulifolius.Methods:The chemical constituents were isolated and purified by chromatography on macroporous resin AB-8,silica gel,ODS,Sephadex LH-20 and prep-HPLC.Their structures were identified by 1H-NMR,13C-NMR and other related spectroscopic methods.Results:Sixteen compounds were isolated from Sauropus spatulifolius and identified as 2,4-di-tert-butylphenol(1),3,5-ditert-butyl-4-hydroxyl phenylpropionic acid hexylester(2),p-hydroxybenzoic acid(3),N-hydroxyethyl-2-acetylpyrrole(4),3-methoxy-4-hydroxybenzoic acid(5),caffeic acid(6),(-)-lyoniresinol-3α-O-β-D-glucopyranoside(7),p-hydroxycinnamic acid(8),3-methoxy-4-hydroxycinnamic acid(9),7-megastigmene-3,6,9-triol(10),7-megastigmane-3,9,10-triol(11),N-(3-carboxypropyl)-2-acetylpyrrole(12),quercetin-3-O-β-D-glucoside(13),3-O-β-glucosyl-(1→6)-β-D-glucosyl-quercetin(14),kaempferol-3-O-β-D-glucoside(15),3-O-β-glucosyl-(1→6)-β-D-glucosyl-kaempferol(16).Conclusion:Compounds 2,3,5,10,11,15 are isolated from the genus for the first time.