不同施肥方式对大豆根际土壤细菌群落多样性的影响

Effects of Different Fertilization Methods on Soil Bacterial Community Diversity in Soybean Rhizosphere

以5个不同施肥处理(T1、T2、T3、T4、T5)下的大豆根际土壤为研究对象,采用Miseq高通量测序技术,分析了不同处理对土壤养分状况和细菌群落多样性的影响。结果表明,T4处理下碱解氮(AN)、有效磷(AP)和速效钾(AK)含量以及地上生物量均最高;综合Shannon指数、Ace指数和Chao1指数3个指标发现,细菌种类在T2、T3、T4处理中较丰富;5个处理的根际土壤细菌被分为379个类群(在属水平上)。土壤细菌相对丰富度和土壤环境因子间的冗余分析表明,AN、AP和AK与交替赤菌属(Alterythrobacter)、假单胞菌属(Pseudomonas)、Pseudarthrobacter相对丰度呈显著正相关。综合分析可知,在复合肥减施20%+有机肥30m^(3)/hm^(2)(T4处理)时,晋豆46号的生物产量达到了最高,而且其优势菌群更有利于土壤培肥和作物增产。

The effects of five distinct fertilization treatments(T1,T2,T3,T4 and T5)on the diversity of the bacterial community and the nutritional status of the soil were investigated using Miseq high-throughput sequencing.The results showed that,the contents of alkali-hydrolyzed nitrogen(AN),available phosphorus(AP),available potassium(AK)and aboveground biomass were the highest with T4 treatment.Based on the three indicators of Shannon index,Ace index and Chao1 index,bacterial species were more abundant in the T2,T3,and T4 treatments.At the genus level,five treated rhizosphere soil bacteria were divided into 379 taxa.Redundancy analysis between relative soil bacterial richness and soil environmental factors showed that,AN,AP and AK showed significant positive correlation with Alterythrobacter,Pseudomonas and Pseudarthrobacter.Comprehensive analysis showed that in the compound fertilizer-organic fertilizer 30m^(3)/ha(T4),the biological yield of Jindou 46 reached the maximum,its dominant flora was more conducive to the soil fertilizer cultivation and crop yield increase.