向日葵盘腐型菌核病诱抗剂筛选及超敏蛋白应用技术研究

Screening of Inducers for Sunflower Sclerotinia sclerotiorum and Application of Hypersensitive Protein

为探明不同植物诱抗剂对向日葵菌核病的诱抗防病效果,先后对10种诱抗剂开展田间药效试验,筛选出超敏蛋白和几丁聚糖2种对向日葵盘腐型菌核病具有良好防效的诱抗剂,并进一步对超敏蛋白的应用技术开展研究。结果显示,分别在向日葵现蕾期和始花期对花盘施用3%超敏蛋白微粒剂317g/hm^(2)可有效诱导向日葵对菌核病产生抗性,防效52.7%~68.3%,与化学防治(菌核净)无显著差异,是较为理想的向日葵菌核病诱抗药剂。同时,2000g/hm^(2)剂量0.5%几丁聚糖水剂的诱抗效果与超敏蛋白无显著差异,亦可用于向日葵菌核病的防治。

In order to explore the effects of different plant inducers on sunflower sclerotiniose,field efficacy tests were carried out on ten inducers successively,two inducers with good control effect on sunflower sclerotiniose were selected,and the application technology of hypersensitive protein was further studied.The results showed that,317g/ha of 3%hypersensitive protein MG applied to the flower plate twice at the budding stage and the initial flowering stage of sunflower could effectively induce sunflower resistance to Sclerotinia sclerotiorum,with a control effects of 52.7%-68.3%,which was not significantly different from chemical control(dimethachlon),so it was an ideal inducer of resistance to S.sclerotiorum.At the same time,the inducing effects of 2000g/ha dose of 0.5%chitosan and hypersensitive protein did not differ significantly.Sunflower sclerotinia can also be treated and prevented with it.