摘要
豌豆(Pisum sativum)是我国重要的豆类经济作物,病害对豌豆生产造成重大经济损失。通过形态学观察、分子鉴定以及致病性测定,最终确定引起豌豆茎基腐病的3种病原菌分别为尖孢镰刀菌(Fusarium oxysporum)、芸苔链格孢菌(Alternaria brassicae)和格氏镰刀菌(F.grosmichelii),优势菌株为尖孢镰刀菌,分离率为53.6%。室内毒力测定结果表明,5种供试杀菌剂对3种病原菌的菌丝生长均有抑制作用,其中咯菌腈和戊唑醇的抑菌效果最好。研究结果为豌豆茎基腐病的防治提供了科学依据。
Pea(Pisum sativum)is one of the most important economic crops of beans in China.The occurrence of diseases will cause great economic losses in pea production.Through morphological observation,molecular identification and pathogenicity determination,the results showed that the three kinds of pathogenic fungi causing pea crown rot were Fusarium oxysporum,Alternaria brassicae and F.grosmichelii,among which F.oxysporum was the most pathogenic,with the isolation frequency of 53.6%.The results of indoor toxicity measurement showed that five fungicides had inhibitory effects on the mycelial growth of three kinds of pathogenic bacteria,among which,the inhibition effect of fludioxonil and tebuconazole have top results.The results provide a scientific basis for the prevention and control of pea crown rot.
作者
宋雅静
欧晋稳
张古文
冯志娟
卜远鹏
王斌
龚亚明
徐建强
刘娜
Yajing Song;Jinwen Ou;Guwen Zhang;Zhijuan Feng;Yuanpeng Bu;Bin Wang;Yaming Gong;Jianqiang Xu;Na Liu(College of Horticulture and Plant Protection,Henan University of Science and Technology,Luoyang 471003,China;Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China,Ministry of Agriculture and Rural Affairs,State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Institute of Vegetables,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;College of Horticulture Science,Zhejiang Agriculture and Forestry University,Hangzhou 311300,China)
出处
《植物学报》
CAS
CSCD
北大核心
2023年第1期132-139,共8页
Chinese Bulletin of Botany
基金
浙江省基础公益研究计划(No.LGN20C150006)
浙江省“十四五”蔬菜新品种选育重大科技专项(No.2021C02065-6-2)
国家自然科学基金(No.31872114)
关键词
豌豆
茎基腐病
分离与鉴定
杀菌剂
敏感性测定
pea
crown rot
isolation and identification
fungicides
sensitivity determination
