内生真菌种类和母本基因型对内生真菌-禾草共生体叶形状和叶面积的影响

Effects of endophyte fungal species and host plant genotype on the leaf shape and leaf area of endophyte-grass symbionts

Epichlo?内生真菌感染能够影响宿主植物的生长发育,但关于内生真菌感染对宿主植物叶形状和叶面积的研究很少。该研究以羽茅(Achnatherum sibiricum)为实验材料,采用长宽系数计算和扫描测定叶面积相结合的方法探究内生真菌种类和羽茅母本基因型对羽茅-内生真菌共生体叶形状和叶面积的影响。结果表明:内生真菌感染与否、内生真菌种类和宿主母本基因型对反映叶形状的叶校正系数、叶片长度、宽度和长宽比均无显著影响,经计算与验证,确定了羽茅叶片的校正系数为0.5949。采用该校正系数及叶长宽计算的叶面积与实测叶面积无显著差异,且二者均未受到内生真菌感染与否、内生真菌种类或宿主植物母本基因型的显著影响。

Aims Epichlo?infection can play an important role in the growth and development of host plants.However,few studies have been reported on the effects of endophyte infection on leaf shape and area of host plants.Methods Our study used Achnatherum sibiricum as experimental material,and explored the effects of endophyte species and host plant genotype on the leaf shape and area of A.sibiricum-endophyte symbionts through the combination of the length and width method with the revised correction coefficient and the scanning calculation method.Important findings Endophyte infection status,endophyte species and host plant genotype had no significant influences on the correction coefficient of leaf size calculation,leaf length,leaf width and leaf length-width ratio reflecting leaf shape.By calculation and verification,we determined that the correction coefficient of leaf size calculation of A.sibiricum was 0.5949.There was no significant difference between the leaf areas measured by length and width method with the revised correction coefficient and the scanning calculation method.In addition,the leaf areas measured by two methods were not significantly affected by endophyte infection,endophyte species and plant maternal genotype.