‘阳光玫瑰’葡萄病毒小RNA测序鉴定及RT-PCR检测

Small RNA sequencing and RT-PCR detection of viruses infecting ‘Shine Muscat’ grapevines

‘阳光玫瑰’是我国从日本引进的葡萄优良品种。为了明确我国‘阳光玫瑰’葡萄病毒病的病原,本研究采用小RNA测序技术对2株显症和无症状的‘阳光玫瑰’葡萄样品进行病毒鉴定结果显示:显症样品中测定到8种病毒,其中包含葡萄蚕豆萎蔫病毒(Grapevine fabavirus,GFabV)和灰比诺葡萄病毒(Grapevine Pinot gris virus,GPGV);无症状样品中测定到3种葡萄病毒。对46个‘阳光玫瑰’样品进行14种葡萄病毒的RT-PCR检测,结果表明:‘阳光玫瑰’葡萄带毒率较高,病毒复合侵染情况普遍;显症样品中,GFabV检出率为88.2%,GPGV和葡萄浆果内坏死病毒(Grapevine berry inner necrosis virus,GINV)检出率为64.7%和29.4%,均明显高于无症状样品(13.8%和10.3%)。本研究旨在探明‘阳光玫瑰’葡萄携带病毒的种类和侵染状况,为其病毒病防控及病毒脱除奠定基础。

‘Shine-Muscat’grapevine is a new introduced cultivar in China.To identify the related viruses with the‘Shine-Muscat’grapevine virus disease,we adopted small RNA sequencing to detect the viruses in the asymptomatic and symptomatic‘Shine-Muscat’grapevine samples.Results showed that the presence of eight viruses in the symptomatic‘Shine-Muscat’grapevine including Grapevine fabavirus(GFabV)and Grapevine Pinot gris virus(GPGV),and only three viruses in the asymptomatic grapevine.Forty-six‘Shine-Muscat’grapevine samples were tested for 14 viruses by RT-PCR,and the results showed that these samples had high virus detection rate,and mixed-infection of multiple viruses was common.Grapevine fabavirus(GFabV)was only found in the symptomatic samples with a detection rate of 88.2%.There was a higher incidence of GPGV and Grapevine berry inner necrosis virus(GINV)(64.7%and 29.4%)in symptomatic samples than that in asymptomatic samples(13.8%and 10.3%).This study identified the species and infection status of viruses in‘Shine-Muscat’grapevines,and provides the theoretical basis for the control of‘Shine-Muscat’grapevine virus disease by sanitary treatments for propagation materials.