硫代乙酰胺所致急性肝损伤大鼠肠道菌群的变化

Research on changes of intestinal flora in rats with acute liver injury induced by thioacetamide

目的探讨硫代乙酰胺所致急性肝损伤大鼠肠道菌群结构、丰度与多样性的变化。方法16只SD大鼠随机分为造模组及正常对照组,造模组大鼠皮下注射硫代乙酰胺600 mg/kg,48小时后造模完成。大鼠经麻醉后,留取肝脏组织进行肝脏病理检测,留取回肠粪便进行肠道菌群16S rDNA测序分析。结果造模组大鼠肠道菌群丰度及多样性较对照组显著升高(P<0.05)。门水平上,两组的优势菌门均为厚壁菌门、拟杆菌门,造模组中依次占61.25%、32.48%,对照组分别占72.50%、25.80%,差异无统计学意义(P>0.05);肠道菌群构成差异分析,造模组变形菌门、疣微菌门菌群与对照组相比,差异有统计学意义(P<0.05);属水平肠道菌群构成差异分析,造模组埃希菌属、阿克曼属、罗马尼亚梭菌属、真菌属及严格梭菌属丰度均高于对照组(P<0.05),乳杆菌属丰度低于对照组(P<0.05)。结论硫代乙酰胺所致急性肝损伤大鼠存在小肠细菌过度生长,变形菌门、埃希菌属丰度升高,乳杆菌属丰度明显下降,存在肠道菌群结构失衡。

Objective To investigate changes of intestinal flora in rats with acute liver injury induced by thioacetamide.Methods Sixteen SD rats were randomly divided into model group and normal control group with 8 rats in each group.Rats in the model group were subcutaneously injected with 600 mg/kg thioacetamide.Rats in the control control group were subcutaneously injected with the same dose of saline.The model was completed48 hours later.Rat feces were collected for 16 S rDNA sequencing of intestinal flora.Results The abundance and diversity of intestinal flora in the model group were significantly higher than those in the control group(P<0.05).At the level of phylum,the dominant phylum of the two groups were Firmicutes and Bacteroidetes,accounting for 61.25%and 32.48%respectively in the model group,72.50%and 25.80%in the control group,but it was no statistical significance(P>0.05).The difference analysis of intestinal flora composition showed that the difference of Proteobacteria and Verrucomicrobia between the model group and the control group was statistically significant(P<0.05).At the level of genus,the abundance of Eubacterium,Akkermansia,Escherichia-Shigella,Ruminiclostridium and Clostridium_sensu_stricto in the model group was higher than that in the control group(P<0.05),and the abundance of Lactobacillus was lower than that in the control group(P<0.05).Conclusion The diversity,composition and abundance of intestinal flora of rats with acute injury caused by thioacetamide were significantly different from those of rats in the control group.There was obvious imbalance of intestinal flora structure.