不同条件微波暴露对冠状病毒灭活效果的定量研究

Quantitative study on inactivation of coronavirus by microwave exposure under different conditions

目的采用定量分析方法评价不同条件微波暴露对小鼠肝炎病毒(冠状病毒研究的模式病毒)的灭活效果。方法采用9.35 GHz不同平均功率密度(200 mW/cm^(2)、100 mW/cm^(2)和50 mW/cm^(2))的微波辐射小鼠肝炎病毒45 min;采用不同暴露时间(45 min、30 min和15 min)的200 mW/cm^(2),9.35 GHz微波辐射小鼠肝炎病毒,通过小鼠成纤维细胞17Cl-1染毒实验及半数细胞感染剂量,评价小鼠肝炎病毒的灭活效果。对于未完全灭活的病毒悬液采用终点稀释法定量计算病毒感染滴度。结果9.35 GHz平均功率密度100 mW/cm^(2),暴露时间45 min可致小鼠肝炎病毒悬液全部灭活,9.35 GHz平均功率200 mW/cm^(2)暴露时间15 min、30 min和45 min均可灭活小鼠肝炎病毒。结论平均功率密度不小于100 mW/cm^(2)的9.35 GHz暴露时间45 min及照射时间不低于15 min的200 mW/cm^(2)的9.35GHz暴露条件均可导致肝炎病毒灭活。

Objective To investigate the inactivation effect of microwave exposure on mouse hepatitis virus(MHV)that is a model virus for coronavirus under different conditions by quantitative analysis.Methods Mice hepatitis virus was irradiated with 9.35 GHz microwave with different average power densities(200 mW/cm^(2),100 mW/cm^(2)and 50 mW/cm^(2))for 45 min.MHV was irradiated with 200 mW/cm^(2)9.35 GHz microwave for different exposure times(45 min,30 min and 15 min).The inactivation effect of MHV was evaluated by the infection experiment of mouse fibroblast 17Cl-1 and 50%tissue culture infectious dose.The titer of virus infection was quantified by endpoint dilution method for incomplete inactivated virus suspension.Results Exposure to 100 mW/cm^(2)of 9.35 GHz power density for 45 min could inactivate all the mouse hepatitis virus suspension,and exposure time of 200 mW/cm^(2)of 9.35 GHz power for 15 min,30 min and 45 min could inactivate MHV.Conclusions 45 min micro⁃wave exposure at 9.35 GHz with average power density not less than 100 mW/cm^(2)and no less than 15 min exposure at 9.35 GHz with average power density of 200 mW/cm^(2)could lead to the inactivation of MHV.