宫颈鳞癌患者组织中ELF5、Ki67、P53表达特征及与疾病分期、预后的关联性研究

Expression characteristics of ELF5,Ki67 and P53 in tissues of patients with cervical squamous cell carcinoma and their correlation with disease stage and prognosis

目的探讨宫颈鳞癌患者组织中ELF5、Ki67、P53表达特征及与疾病分期、预后的关联性。方法回顾性选取2019年5月—2021年9月新疆生产建设兵团医院宫颈鳞癌患者30例设为宫颈鳞癌组、低级别CIN患者20例设为低级别CIN组、高级别CIN患者20例设为高级别CIN组、慢性宫颈炎患者20例设为慢性宫颈炎组。采取免疫组织化学法测定各个组织内P53、Ki67、ELF5表达情况,统计以上4组宫颈组织中P53、Ki67、ELF5阳性表达情况,分析上述指标与宫颈鳞癌临床病理指标的关系,并比较不同预后的宫颈鳞癌患者P53、Ki67、ELF5阳性率。结果(1)宫颈鳞癌患者ELF5阳性表达率(33.33%)低于低级别CIN组(85.00%)、高级别CIN组(75.00%)、慢性宫颈炎组(90.00%),P53、Ki67阳性表达率(76.67%、83.33%)高于低级别CIN组(20.00%、25.00%)、高级别CIN组(45.00%、55.00%)、慢性宫颈炎组(5.00%、5.00%)(P<0.05);(2)不同FIGO分期宫颈鳞癌者P53、Ki67、ELF5阳性表达情况比较(P<0.05),且随着FIGO分期增高,ELF5阳性表达率持续降低,P53、Ki67阳性表达率持续增高(P<0.05);(3)P53、Ki67阳性表达率与宫颈鳞癌FIGO分期间存在显著正相关关系,ELF5阳性表达率与宫颈鳞癌FIGO分期间存在显著负相关关系(P<0.05);(4)预后不良者ELF5阳性表达率(10.53%)低于预后良好者(72.73%),P53、Ki67阳性表达率(94.74%、100.00%)高于预后良好者(45.45%、45.45%)(P<0.05)。结论宫颈鳞癌组织中P53、Ki67、ELF5表达情况异常增高,其增高幅度与疾病分期具有密切关联性,且不同预后情况者上述指标表达间存在显著差异,其可用于疾病预后的预测。

Objective To investigate the expression characteristics of ELF5,Ki67 and P53 in cervical squamous cell carcinoma(CSC)and their correlation with disease stage and prognosis.Methods From May 2019 to September 2021,30 patients with cervical squamous cell carcinoma in Xinjiang Production and Construction Corps Hospital hospital were retrospectively selected as cervical squamous cell carcinoma group,20 patients with low-grade CIN as low-grade CIN group,20 patients with high-grade CIN as high-grade CIN group,and 20 patients with chronic cervicitis as chronic cervicitis group.Immunohistochemical method was used to determine the expression of P53,Ki67 and ELF5 in each tissue,and the positive expression of P53,Ki67 and ELF5 in the above four groups of cervical tissues was counted.The relationship between the above indicators and the clinicopathological indicators of cervical squamous cell carcinoma was analyzed,and the positive rates of P53,Ki67 and ELF5 in patients with cervical squamous cell carcinoma with different prognosis were compared.Results(1)The positive expression rate of ELF5(33.33%)in cervical squamous cell carcinoma patients was lower than that in low-grade CIN group(85.00%),high-grade CIN group(75.00%),and chronic cervicitis group(90.00%).The positive expression rates of P53 and Ki67(76.67%,83.33%)were higher than that in low-grade CIN group(20.00%,25.00%),high-grade CIN group(45.00%,55.00%),and chronic cervicitis group(5.00%,5.00%)(P<0.05).(2)The positive expression of P53,Ki67 and ELF5 in cervical squamous cell carcinoma patients at different FIGO stages was compared(P<0.05).With the increase of FIGO stages,the positive expression rate of ELF5 continued to decrease,while the positive expression rates of P53 and Ki67 continued to increase(P<0.05).(3)There was a significant positive correlation between P53,Ki67 positive expression rate and FIGO stage of cervical squamous cell carcinoma,and a significant negative correlation between ELF5 positive expression rate and FIGO stage of cervical squamous cell carcinoma(P<0.05).(4)The positive expression rate of ELF5 in patients with poor prognosis(10.53%)was lower than that in patients with good prognosis(72.73%),and the positive expression rates of P53 and Ki67(94.74%,100.00%)were higher than those in patients with good prognosis(45.45%,45.45%)(P<0.05).Conclusion The expression of P53,Ki67 and ELF5 in cervical squamous cell carcinoma is abnormally high,which is closely related to the disease stage,and there are significant differences between the expression of the above indicators in patients with different prognosis,which can be used to predict the prognosis of the disease.