摘要
Objective:The goal of this study was to investigate the effect of anticoagulant intervention on inflammatory factors in rats with chronic obstructive pulmonary disease(COPD)and its correlation with thrombus factors.Methods:Sixty Wistar rats were randomly assigned to one of three groups:model group(n=20),anticoagulation intervention group(n=20),and healthy control group.The rats in the healthy control group were fed normally,with no special treatment.The rats in the model group and the anticoagulation intervention groups had COPD models created by cigarette smoking combined with intratracheal injection of lipopolysaccharide.Following successful modeling,rats in the model group received a subcutaneous injection of normal saline,while the rats in the anticoagulation intervention group received a subcutaneous injection of low molecular weight heparin sodium.The three groups were compared in terms of lung histomorphology,pulmonary function measures,serum levels of inflammatory and thrombotic markers.The link between inflammatory variables and thrombus components in COPD model rats was determined using Pearson correlation analysis.Results:The mean lining interval(MLI)and the ratio of alveolar area to total lung area(PAA)in the model group and the anticoagulation groups were larger than those in the healthy control group,whereas the mean alveolar number(MAN)was lower.MLI and PAA were lower in the anticoagulation intervention group than in the model group,whereas MAN was higher in the model group(P<0.05).Tidal volume,minute ventilation,and FEV1/FVC were lower in the model and anticoagulation intervention groups than in the healthy control group,but lung compliance was higher in the model and anticoagulation intervention groups.Tidal volume,minute ventilation volume,and FEV1/FVC were higher in the anticoagulation intervention group than in the model group,whereas lung compliance was worse in the model group(P<0.05).The serum levels of tumor necrosis factor-α(TNF-α),interleukin-8(IL-8)and interleukin-6(IL-6)in the model and anti-coagulation intervention groups were higher than those in the healthy control group,but lower in the anti-coagulation intervention group than in the model group(P<0.05).The serum levels of tissue factor(TF)and tissue factor pathway inhibitor-1(TFPI-1)were higher in the model group and anticoagulation intervention groups than those in the healthy control group,while the levels of the above thrombotic factors were lower in the anticoagulation intervention group than those in the model group(P<0.05).Pearson correlation analysis revealed that TNF-α,IL-8 and IL-6 levels were favorably linked with TF and TFPI-1 levels in COPD model rats(P<0.05).Conclusion:Anticoagulant therapy can successfully enhance pulmonary function in COPD rats,while also negatively regulating inflammatory and thrombus factors,and there is a strong link between inflammatory factors and thrombus factors.
