植物中总黄酮检测方法研究进展

Research Progress on Detection Methods of Total Flavonoids in Plants

黄酮是植物中重要的一类次级代谢产物,在植物体内大部分与糖结合成苷类或碳糖基的形式存在,也有以游离形式存在的。黄酮类化合物具备抗氧化、抗菌以及抗病毒等多种生物活性,被大量应用于药品、食品、化妆品、畜禽饲料添加剂等行业,不同于人工合成添加剂混有有害杂质或用量过大时容易造成对机体的危害,其作为天然添加剂,不仅提高了食品的质量和安全性,还降低了环境污染。本文阐述了植物中总黄酮提取和测定方法,并进行了分类统计,分析各种方法的优缺点,对总黄酮测定存在的问题和今后的研究方向提出建议,为开展相关研究的科研工作者提供参考。

Flavonoids are an important class of secondary metabolites in plants,which mostly exist in the form of glycosides or carbohydrates synthesized by glycosylation,as well as in free form.Flavonoids have various biological activities such as antioxidant,antibacterial,and antiviral pr operties,and are widely used in industries such as pharmaceuti cals,food,cosmetics,and animal feed additives.As natural additives,flavonoids are different from artificially synthesized additives that contain harmful impurities or easily cause harm to the body when used in excessive amounts.They can not only improve the quality and safety of food,but also reduce environmental pollution.This article elaborates on the extraction and determination methods of total flavonoids from plants,conducts classification statistics,analyzes the advantages and disadvantages of various methods,and proposes suggestions for the problems and future research directions of total flavonoid determination,providing reference for future researchers in re lated research.