磁场对柴胡种子萌发及幼苗抗逆性的影响

Effects of Magnetic Field on Seed Germination and Seedling Stress Resistance of Bupleurum chinense

研究不同强度、不同作用时间的磁场处理对柴胡种子萌发及幼苗抗逆性的影响,为柴胡人工栽培提供科学依据。采用双层滤纸培养法,将处理后的柴胡种子置于25℃恒温40%光照的培养箱中培养,观察柴胡种子的发芽率、发芽势、株高,测定幼苗幼叶中叶绿素含量、CAT、POD、SOD酶活性。多数经磁场处理的柴胡种子发芽率、发芽势及幼叶中的CAT、POD、SOD活性高于对照,100 mT 55 min处理的发芽率、POD、SOD活性最高。柴胡种子放置时间越长,种子活力越低。适当的磁场处理,能显著促进柴胡种子的萌发,提高柴胡幼苗的抗逆性,对柴胡的人工栽培有一定的指导意义。

The effects of magnetic field with different intensities and different processing time on seed germination and seedling stress resistance of Bupleurum chinense were studied in order to provide a basis for artificial cultivation of B.chinense.Adopted a double-layer filter paper culture method,the treated seeds were cultured in the incubator at 25℃with 40%light.The germination rate,germination energy and the shoot height of the seedling were recorded.The physiological indexes such as chlorophyll content and enzymatic activities of CAT,POD and SOD in leaves were measured.The germination rate,germination energy,enzymatic activities of CAT,POD and SOD in most treated seeds and seedlings were higher than that in the control,the germination rate and enzymatic activity of POD and SOD were the highest under the condition of 100 mT,55 min.Meanwhile,the seed vigor decreased with the prolonged store time of the seeds.The proper magnetic field can significantly promote seed germination and stress resistance of B.chinense,which is of great significance to artificial cultivation of B.chinense.