蛹虫草子实体多糖提取工艺及其体外抗氧化性研究

Extraction Technology and Antioxidant Activity of Polysaccharides from Cordyceps militaris Fruiting Body

为得到最优的热水浸提蛹虫草子实体多糖的条件以及为后期的纯化、免疫活性研究提供参考依据,以蛹虫草子实体为研究对象,采用新脱蛋白方法,在单因素试验的基础上,利用响应面法对蛹虫草子实体多糖提取工艺参数进行优化研究,并对该多糖进行抗氧化活性研究。响应面优化的最佳提取工艺为浸提温度80℃,浸提时间3.1 h,液料比41:1(mL:g),浸提次数2次。此条件下多糖实际得率为6.3328%,与预测值6.5877%的相对误差小于5%,表明该提取工艺优化参数较为可靠。采用该方法获得的多糖在8 mg/mL时,羟自由基清除能力高达53.8%,铁离子还原力高达1.133,总抗氧化性达0.807;在6 mg/mL时,DPPH自由基清除能力最强,IC50为1.449 mg/mL。试验获得了多糖高提取率、高抗氧化性的提取方法,为后期蛹虫草子实体多糖的分离、纯化、生物活性研究提供参考。

The paper aims to obtain the optimal condition for extracting polysaccharide from Cordyceps militaris fruiting body by hot water and to provide reference for the study of its immune activity.Taking C.militaris fruiting body as the research object,using the new deproteinization method,based on the single factor experiment and the response surface method,we optimized the extraction process parameters of the polysaccharide of C.militaris fruiting body,and studied the antioxidant activity of the polysaccharide.The optimum extraction condition is:the extraction temperature of 80℃,the extraction time of 3.1 h,the ratio of liquid to material of 41:1(mL:g),and the extraction of 2 times.Under this condition,the actual yield of polysaccharide is 6.3328%,and the relative error from the predicted value of 6.5877%is less than 5%,indicating that the optimization parameters of extraction process are reliable.The scavenging ability of hydroxyl radical is 53.8%,the reduction ability of iron ion is 1.133 and the total antioxidant activity is 0.807 when the polysaccharide obtained by this method is 8 mg/mL.The scavenging ability of 50%inhibiting concentration is 1.449 mg/mL.The method of extraction of polysaccharide with high extraction rate and high antioxidant activity is obtained,which could lay a foundation for the separation,purification and bioactivity of polysaccharide from C.militaris fruiting body.