蒙医希拉病大鼠模型的肠道菌群特征

Characteristics of intestinal flora in rat model of Xila disease in Mongolian medicine

目的:研究揭示蒙医希拉病大鼠模型的肠道菌群特征。方法:将实验大鼠随机分为模型组、对照组,以四施干预法建立希拉病动物模型,记录大鼠体质量、行为学;利用酶联免疫吸附法检测胃泌素(GAS);收集实验大鼠粪便,以高通量测序法分析OTU(operational taxonomic units)组成及肠道微生物;基于肠道菌群β、Alpha多样性分析四施干预法建立蒙医希拉病模型对大鼠肠道菌群的影响;分析差异菌属。结果:在20个样本中,获得670个OTU,检测中拟菌门和乳杆菌门为丰度较高的微生物门。对照组、模型组的Chao指数分别为592.74±25.62和609.71±16.64;Ace指数分别为586.76±23.97和602.08±15.84;Simpson指数分别为9.05×10^(-2)±0.05和2.82×10^(-2)±0.01;Shannon指数分别为3.84±0.51和4.55±0.28。与对照组相比较,模型组Clostridia、Allobaculum、Proteobacteria、Faecalibaculum、Desulfovibrionacteria和Ruminococcaceae等6个菌属丰度显著增加,而Prevotella和Bacilli等4个菌属丰度显著下降,差异均有统计学意义(P<0.05,P<0.01)。结论:蒙医四施干预法建立希拉病大鼠模型后与正常大鼠比较其肠道菌群有显著差异。

Objective:To establish an animal model of Sheila disease based on the three root theories of Mongolian medicine,detect serum GAS,analyze and analyze the pathophysiological characteristics of Sheila disease by metabonomics.Based on the clinical characteristics and symptoms of Sheila disease,an animal model wasestablished using four intervention methods,and evaluated from multiple perspectives including enzyme linked immunosorbent assay,metabolomics,and behavioral observation.Methods:SD rats were randomly divided into control group and model group,and their body mass and behavior were recorded;Gastrin(GAS)was detected by enzyme linked immunosorbent assay;The differential metabolites in serum were detected using metabonomic methods.Results:There was no significant difference in body mass between the model groups measured by behavioral tests;The level of GAS in the control group was significantly higher than that in the model group measured by ELISA;According to metabolomic data statistics,there are mainly 8 metabolic pathways in Sheila disease,including purine metabolism,pyrimidine metabolism,biosynthesis of plant secondary metabolites,metabolism of nicotinic acid and nicotinamide,biosynthesis of phenylpropanoid compounds,biosynthesis of tropine,piperidine,and pyridine alkaloids,biosynthesis of phenylpropane,and biosynthesis of ornithine,lysine,and nicotinic acid alkaloids.Conclusion:The animal model of Sheila disease established by the four intervention methods of Mongolian medicine is consistent with the theory of Sheila disease,and the pathogenesis of Sheila disease may be related to the biosynthesis of purine metabolism,pyrimidine metabolism,plant secondary metabolites,nicotinic acid and nicotinamide metabolism,phenylpropane compounds biosynthesis,Tropane,piperidine,and pyridine alkaloids biosynthesis,phenylpropane biosynthesis,ornithine,lysine,and nicotinic acid alkaloids biosynthesis pathways.