卡托普利预处理对自酸蚀粘接剂牙本质粘接持久性的影响

Effect of captopril pretreatment on the dentin bonding durability of self-etch adhesive

目的:通过卡托普利抑制金属基质蛋白酶的活性以提高粘接界面的稳定性。方法:采用不同浓度的卡托普利乙醇溶液和卡托普利乙醇/水溶液对人离体牙牙本质表面进行预处理,通过场发射扫描电镜观察卡托普利预处理对牙本质表面形貌的影响;在此基础上通过两种自酸蚀粘接剂对牙本质进行粘接,表征即刻粘接强度,并通过老化处理,评价卡托普利预处理对粘接持久性的影响。结果:扫描电镜结果提示,卡托普利溶液可以部分去除沾污层并暴露牙本质胶原。基于扫描电镜结果,选择0.15 g/m L卡托普利乙醇溶液(50%乙醇)处理30 s作为预处理条件,通过可乐丽菲露SE BOND进行牙本质粘接,即刻微拉伸强度从(30.80±4.70)MPa提升至(37.48±3.20)MPa(P<0.05);老化处理1年后,对照组微拉伸强度显著下降[(22.90±6.82)MPa,P<0.05],而实验组微拉伸强度没有明显的变化[(36.56±5.10)MPa,P>0.05]。用可乐丽菲露S3BOND进行牙本质粘接,对照组的即刻微拉伸强度为(31.33±4.11)MPa,与实验组(34.70±4.07)MPa差异无统计学意义(P>0.05);老化处理1年后,实验组的微拉伸强度高于对照组[分别为(32.36±3.58)MPa和(21.43±6.27)MPa,P<0.05]。结论:卡托普利预处理不仅可以提高自酸蚀粘接的即刻粘接强度,而且改善了粘接的持久性。

Objective:To investigate the effect of captopril on the dentin bonding durability of self-etch adhesive.Methods:Different concentrations of captopril ethanol solutions or captopril ethanol/water solutions were prepared to pretreat dentin as primer for the self-etch adhesives.The surface morphology of the dentin was observed with scanning electron microscopy(SEM).Based on the morphology analysis,the pretreatment condition was selected and two self-etch adhesives were employed to evaluate the improvement effect of the captopril pretreatment on the dentin bonding durability.Results:SEM showed that the pretreatment of captopril ethanol solutions and captopril ethanol/water solutions were able to remove the smear lay and partially expose collagen matrix.According to the SEM results,the pretreating condition of 0.15 g/mL captopril ethanol/water solution with the pretreating time of 30 s was selected for further dentin bonding study.For Clearfil SEBOND system,the immediate bonding strength increased from(30.80±4.70)MPa to(37.48±3.20)MPa(P<0.05).After one-year aging,the bonding strength of the control group decreased markedly[(22.90±6.82)MPa,P<0.05];while the bonding strength of the captopril pretreated group kept steadily[(36.56±5.10)MPa,P>0.05].For Clearfil S3 BOND system,there was no significant difference in the immediate bonding strength between the experimental group[(34.70±4.07)MPa]and the control group[(31.33±4.11)MPa].But after one-year aging,the bonding strength of the experimental group was higher than that of the control group[(32.36±3.58)MPa and(21.43±6.27)MPa,P<0.05].Conclusion:The pretreatment with captopril ethanol/water solution increases the dentin bonding strength of the self-etch adhesive systems and also improves the bonding durability.