喂养方式对腹泻患儿头孢克洛使用前后肠道菌群的影响

Effect of feeding methods on intestinal flora of children with diarrhea before and after cefaclor use

目的探讨母乳喂养和配方奶喂养对腹泻患儿头孢克洛使用前后肠道菌群的影响。方法选择浙江省杭州市中医院2018年1-10月60例6个月-2岁腹泻患儿20例,母乳组和配方组各10例,搜集腹泻1天内(DI)、治疗后3天(DIN)、治愈后3个月(DIR)共60份粪便样本。利用16S rDNA测序技术分析两组不同时间段微生物丰度多样性及差异性。结果使用头孢克洛后,肠道菌群中厚壁菌门下降明显,且在停药3个月后菌群结构与治疗过程中各优势菌丰度及多样性无明显差异;停药后3个月母乳组比配方组菌群厚壁菌门丰度增加,多样性有明显差异。结论母乳组腹泻患儿在服用抗菌药物后肠道稳定性较配方组高,减少抗菌药物使用、提倡母乳喂养可优化婴幼儿肠道菌群的结构。

Objective To investigate the changes of intestinal microflora in infants with diarrhea under antibiotic administration,and the effects of different feeding methods on intestinal microflora.Methods The fecal samples of 60 diarrhea infants aged 6 months to 2 years from January 2018 to October 2018 were selected,and the samples were divided into 2 groups:breast-feeding and formula milk feeding,16 s r RNA sequencing technology was used to analyze the abundance and diversity of microorganisms in each group and analyze their differences.Results Firmicutes in intestinal flora decreased significantly after treatment with cefacroxan,and there was no significant difference in the abundance and diversity of dominant bacteria after 3 months of treatment with cefacroxan.The abundance of Firmicutes in the breast-feeding group was high-er than that in the formula group at 3 months after drug withdrawal,and the diversity was significantly different.Conclusion The use of antibiotics has a long-term impact on the diversity of intestinal flora in infants.Breast-fed children with diarrhea have higher intestinal stability than formula milk group.Reducing the use of antibiotics and promoting breastfeeding can op-timize the intestinal structure of infants.