红景天苷调节FAK/MEK/ERK信号通路对HPV阳性宫颈癌细胞发生发展的影响

Effect of salidroside on the occurrence and development of HPV positive cervical cancer cells by regulating the FAK/MEK/ERK signaling pathway

目的探讨红景天苷(Sal)调节局部黏着斑激酶(FAK)/胞外信号调节激酶的激酶(MEK)/细胞外信号调节激酶(ERK)信号通路对人乳头瘤病毒(HPV)阳性宫颈癌细胞发生发展的影响。方法分别用0、2.5、5、10、20、40、80、160、320μg/mL Sal处理HPV18阳性人宫颈癌细胞HeLa,根据细胞存活率确定适宜的Sal浓度用于正式实验。将HeLa细胞分组为对照组、Sal低剂量组、Sal中剂量组、Sal高剂量组、表皮细胞生长因子(EGF)(FAK激活剂)组、Sal高剂量+EGF组,CCK-8法、5-乙炔基-2′-脱氧尿苷(EdU)染色检测细胞增殖;Transwell检测细胞迁移与侵袭;qRT-PCR检测HeLa细胞中增殖细胞核抗原(PCNA)、转移侵袭增强因子1(MIEN1)、基质金属蛋白酶9(MMP-9)mRNA表达;Western Blot检测细胞中人乳头瘤病毒18型(HPV18)E6、HPV18 E7、p-FAK、p-MEK、p-ERK1/2蛋白表达。结果选取Sal浓度为40、80、160μg/mL用于正式实验。与对照组比较,Sal低剂量组、Sal中剂量组、Sal高剂量组HeLa细胞A_(450)值、EdU阳性细胞率、细胞迁移及侵袭数目、PCNA、MIEN1、MMP-9 mRNA表达及HPV18 E6、HPV18 E7、p-FAK、p-MEK、p-ERK1/2蛋白表达降低,且呈剂量依赖性(P<0.05);与对照组比较,EGF组HeLa细胞A_(450)值、EdU阳性细胞率、细胞迁移及侵袭数目、PCNA、MIEN1、MMP-9 mRNA表达及HPV18 E6、HPV18 E7、p-FAK、p-MEK、p-ERK1/2蛋白表达升高(P<0.05);与Sal高剂量组比较,Sal高剂量+EGF组HeLa细胞A_(450)值、EdU阳性细胞率、细胞迁移及侵袭数目、PCNA、MIEN1、MMP-9 mRNA表达及HPV18 E6、HPV18 E7、p-FAK、p-MEK、p-ERK1/2蛋白表达升高(P<0.05)。结论Sal可能通过抑制FAK/MEK/ERK信号通路抑制HPV阳性宫颈癌细胞发生发展。

Objective To investigate the effects of salidroside(Sal)on the occurrence and development of human papillomavirus(HPV)positive cervical cancer cells by regulating the focal adhesion kinase(FAK)/mitogen-activated protein kinase(MEK)/extracellular regulated protein kinase(ERK)signaling pathway.Methods HPV18 positive human cervical cancer cells HeLa were treated with 0,2.5,5,10,20,40,80,160,and 320μg/mL Sal,respectively.The appropriate Sal concentration was determined based on cell survival rate for formal experiments.HeLa cells were divided into control group,low-dose Sal group,medium-dose Sal group,high-dose Sal group,epidermal growth factor(EGF)(FAK activator)group,and high-dose Sal+EGF group,CCK-8 method and 5-ethynyl-2'-deoxyuridine(EdU)staining were applied to detect cell proliferation;Transwell was applied to detect cell migration and invasion;qRT-PCR was applied to detect the mRNA expression of proliferating cell nuclear antigen(PCNA),migration and invasion enhancer 1(MIEN1),and matrix metalloproteinase-9(MMP-9)in HeLa cells;Western blot was applied to detect the expression of human papillomavirus 18(HPV18)E6,HPV18 E7,p-FAK,p-MEK,and p-ERK1/2 proteins in cells.Results Sal concentrations of 40,80,and 160μg/mL were selected for formal experiments.Compared with the control group,the A_(450) value,EdU positive cell rate,cell migration and invasion numbers,PCNA,MIEN1,MMP-9 mRNA expression,and HPV18 E6,HPV18 E7,p-FAK,p-MEK,p-ERK1/2 protein expression of HeLa cells in the low-dose,medium-dose,and high-dose Sal groups were reduced,and were dose-dependent(P<0.05);compared with the control group,the A_(450) value,EdU positive cell rate,cell migration and invasion numbers,PCNA,MIEN1,MMP-9 mRNA expression,and HPV18 E6,HPV18 E7,p-FAK,p-MEK,and p-ERK1/2 protein expression of HeLa cells in the EGF group were increased(P<0.05);compared with the high-dose Sal group,the A_(450) value,EdU positive cell rate,cell migration and invasion numbers,PCNA,MIEN1,MMP-9 mRNA expression,and protein expression of HPV18 E6,HPV18 E7,p-FAK,p-MEK,and p-ERK1/2 in HeLa cells in the high-dose Sal+EGF group were increased(P<0.05).Conclusion Sal may inhibit the occurrence and development of HPV positive cervical cancer cells by inhibiting the FAK/MEK/ERK signaling pathway.