细菌性脑膜炎患者脑脊液、血清lncRNA NEAT1水平与病情及血脑屏障破坏程度相关性分析

Correlation of lncRNA NEAT1 level in cerebrospinal fluid and serum with disease severity and blood-brain barrier damage in patients with bacterial meningitis

目的 检测细菌性脑膜炎(BM)患者脑脊液、血清中lncRNA NEAT1水平变化情况,并分析其与患者病情严重程度以及血脑屏障破坏程度的相关性。方法 收集2015年2月-2021年12月间本院收治的BM患者66例作为BM组,另选取同期不具备神经系统感染性疾病但具有腰穿指征的同期住院患者40例作为对照组。根据中枢神经系统功能障碍程度将患者分为普通BM组与重症BM组,收集患者入院48 h内脑脊液与血清标本,qRT-PCR法检测脑脊液与血清标本中lncRNA NEAT1水平,比较对照组、BM组间以及普通BM组与重症BM组间脑脊液与血清标本中lncRNA NEAT1水平;根据白蛋白指数对BM患者血脑屏障破坏程度进行分级,比较普通BM组与重症BM组血脑屏障破坏程度;Pearson法分析脑脊液lncRNA NEAT1、血清lncRNA NEAT1、白蛋白指数间相关性。结果 与对照组相比,BM组患者脑脊液(3.28±0.51 vs 1.01±0.18)、血清lncRNA NEAT1水平(2.51±0.42 vs 1.01±0.16)均升高(P<0.05),且BM组患者血清与脑脊液中lncRNA NEAT1水平比较差异有统计学意义(P<0.05);与普通BM患者相比,重症BM患者脑脊液lncRNA NEAT1(3.62±0.53 vs 2.82±0.46)、血清lncRNA NEAT1(2.70±0.46 vs 2.25±0.37)水平升高(P<0.05),且普通BM、重症BM患者血清与脑脊液中lncRNA NEAT1水平比较差异有统计学意义(P<0.05);普通BM患者、重症BM患者血脑屏障损伤程度之间比较差异有统计学意义(P<0.05);BM患者脑脊液、血清lncRNA NEAT1均与白蛋白指数呈正相关(P<0.05),BM患者脑脊液lncRNA NEAT1与血清lncRNA 结论 BM患者脑脊液、血清中lncRNA NEAT1高表达,其水平与患者严重程度、血脑屏障损伤程度密切相关,为BM患者的诊疗提供了靶点。

Objective To detect the changes in lncRNA NEAT1 level in cerebrospinal fluid and serum of patients with bacterial meningitis(BM),and to analyze its correlation with the severity of the disease and the degree of blood-brain barrier damage. Methods From February 2015 to December 2021,66 BM patiens admitted to our hospital were regarded as the BM group. Another 40 cases of hospitalized patients who did not have nervous system infectious diseases but had lumbar puncture indications were regarded as the control group. The patients were grouped into normal BM group and severe BM group according to the degree of central nervous system dysfunction. The cerebrospinal fluid and serum samples were collected within 48 hours of admission, and qRT-PCR was used to detect the level of lncRNA NEAT1 in the cerebrospinal fluid and serum samples, the level of lncRNA NEAT1 in cerebrospinal fluid and serum samples was compared between the control group and the BM group, and between the normal BM group and the severe BM group;the degree of blood-brain barrier damage in BM patients was graded according to the albumin index, and the degree of blood-brain barrier damage in the normal BM group and the severe BM group was compared;Pearson’s method was applied to analyze the correlation between cerebrospinal fluid lncRNA NEAT1,serum lncRNA NEAT1 and albumin index. Results Compared with the control group, the level of lncRNA NEAT1 in the cerebrospinal fluid(3.28±0.51 vs 1.01±0.18) and serum(2.51±0.42 vs 1.01±0.16) of patients in the BM group were increased(P<0.05),and the level of lncRNA NEAT1 in serum and cerebrospinal fluid of patients in BM group was obviously different(P<0.05);compared with patients with normal BM,the level of lncRNA NEAT1 in cerebrospinal fluid(3.62±0.53 vs 2.82±0.46) and the level of lncRNA NEAT1 in serum(2.70±0.46 vs 2.25± 0.37) were increased in patients with severe BM(P<0.05),and there was a statistically obvious difference in the level of lncRNA NEAT1 in serum and cerebrospinal fluid between normal BM and severe BM(P<0.05);there was a statistically obvious difference in the degree of blood-brain barrier damage between patients with normal BM and patients with severe BM(P<0.05);the lncRNA NEAT1 in the cerebrospinal fluid and serum of patients with BM were positively correlated with the albumin index(P<0.05),the lncRNA NEAT1 in the cerebrospinal fluid was positively correlated with lncRNA NEAT1 in serum in patients with BM(P<0.05). Conclusion The lncRNA NEAT1 is highly expressed in the cerebrospinal fluid and serum of patients with BM,and its level is closely related to the severity of the patients and the degree of blood-brain barrier damage, which provides a target for the diagnosis and treatment of patients with BM.