肠道益生菌对靶向bFGF的肿瘤疫苗抗小鼠黑色素瘤的影响

Effect of Probiotics on bFGF Antigen Vaccine in Treatment of Murine Melanoma

[目的]探讨肠道益生菌对碱性成纤维细胞生长因子(bFGF)疫苗抗黑色素瘤的影响。[方法]小鼠分别用嗜酸乳杆菌、双歧杆菌、粪肠球菌灌胃,同时皮下注射bFGF抗原进行免疫,每周一次,共免疫5次后,皮下接种B16-F10黑色素瘤细胞,观察对肿瘤生长的抑制和肺转移的影响,接种癌细胞前对抗体的表达、细胞因子的释放、肠道微生物的变化、相关基因的表达分别进行检测。[结果]三种微生物中双歧杆菌能有效增加T细胞活化和DC细胞成熟相关基因的表达,促进IFN-γ的分泌,有效提升细胞免疫应答,加强免疫治疗疗效。[结论]双歧杆菌有助于CD8^+T细胞功能活化,提升机体对抗原的应答,提高免疫治疗疗效,未来临床有一定的潜在应用价值。

[Purpose]To investigate the effect of intestinal probiotics on bFGF antigen vaccine in treatment of murine melanoma.[Methods]Lactobacillus acidophilus,Bifidobacterium and Enterococcus faecalis were given to C57 BL/6 mice by gavage.The mice were subcutaneously injected with bFGF antigen vaccine once a week for 5 weeks,then B16-F10 melanoma cells were inoculated subcutaneously.The inhibition of tumor growth and lung metastasis were observed.The expression of antibodies,the release of cytokines,the changes of intestinal microorganisms and related genes were examined before and after the tumor cell inoculation.[Results]Bifidobacterium increased the expression of genes related to T cell activation and DC cell maturation,promoted the secretion of IFN-γ,enhanced the cellular immune response,and improved the efficacy of immunotherapy.[Conclusion]Bifidobacterium can promote the activation of CD8^+T cells,enhance the response of the tumor bearing mice to bFGF vaccine,and improve the efficacy of immunotherapy,which may have potential clinical application value.