纳米银对人骨髓间充质干细胞增殖和凋亡的影响

Effects of Silver Nanoparticles on Proliferation and Apoptosis of Human Bone Marrow Mesenchymal Stem Cells

目的阐明纳米银(AgNP)对骨髓间充质干细胞(BMSC)增殖和凋亡的影响。方法BMSC分为对照组、AgNP组和Akt抑制剂组。CCK-8、Western blot检测各组BMSCs增殖吸光度值(A值)和Caspase 3蛋白的表达;ELISA检测各组BMSCs裂解液中总超氧化物歧化酶(Total-SOD)和丙二醛(MDA)含量。结果与对照组相比,AgNP组BMSCs的A值明显提高(P<0.01);Caspase 3蛋白表达显著降低(P<0.01);AgNP组Total-SOD表达明显增加(P<0.01),MDA显著降低(P<0.01)。相对于AgNP组,Akt抑制剂组的Total-SOD、MDA表达均呈相反趋势。结论纳米银通过Akt通路促进表达Total-SOD,提高BMSCs增殖,抑制其凋亡。

Objective To explain the effects of silver nanoparticles(AgNP)on proliferation,apoptosis of human bone marrow mesenchymal stem cells(BMSC),and its mechanism.Methods The BMSCs were divided into control group,AgNP group and Akt inhibitor group.The CCK-8 assay and Western blot were used to detect the BMSCs proliferation absorbance value(A value)and Caspase 3 expression in each group,respectively.The ELISA assay was also used to detect the BMSC stotal superoxide dismutase(Total-SOD)and malondialdehyde(MDA)content of each group.Results Compared with the control group,the A value of BMSCs was significantly increased in AgNP group(P<0.01),and expression of Caspase 3 significantly decreased(P<0.01).In AgNP group,the expression of Total-SOD significantly increased(P<0.01),and the expression of MDA significantly decreased(P<0.01).The expression or content of Total-SOD or MDA in the Akt inhibitor group was opposite to that in the AgNP group.Conclusion The activation of Akt signaling pathway by Silver nanoparticles increased the expression of Total-SOD and other antioxidant enzymes,increase the proliferation of BMSCs and inhibit their apoptosis.